Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-couple_hipB
Location 408287..410106 Replicon plasmid pKM12v1
Accession NZ_CP034838
Organism Rahnella aquatilis strain KM12

Toxin (Protein)


Gene name hipA Uniprot ID H8P0A1
Locus tag EJP81_RS24370 Protein ID WP_013578107.1
Coordinates 408757..410106 (-) Length 450 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID H8P0A0
Locus tag EJP81_RS24365 Protein ID WP_013578106.1
Coordinates 408287..408760 (-) Length 158 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EJP81_RS24345 403528..404994 + 1467 WP_168444346.1 HAMP domain-containing histidine kinase -
EJP81_RS24350 405241..406740 + 1500 WP_013578103.1 UbiD family decarboxylase -
EJP81_RS24355 406937..407791 + 855 WP_013578104.1 LysR family transcriptional regulator -
EJP81_RS24360 407907..408227 + 321 WP_013578105.1 multidrug efflux SMR transporter -
EJP81_RS24365 408287..408760 - 474 WP_013578106.1 helix-turn-helix domain-containing protein Antitoxin
EJP81_RS24370 408757..410106 - 1350 WP_013578107.1 type II toxin-antitoxin system HipA family toxin Toxin
EJP81_RS24375 410302..410523 - 222 WP_014416648.1 DUF1471 domain-containing protein -
EJP81_RS24380 410692..411315 - 624 WP_112151436.1 NAD(P)H-binding protein -
EJP81_RS24385 411312..411833 - 522 WP_165497365.1 heme utilization cystosolic carrier protein HutX -
EJP81_RS24390 411811..413136 - 1326 WP_168444347.1 heme anaerobic degradation radical SAM methyltransferase ChuW/HutW -
EJP81_RS24395 413249..413599 - 351 WP_112151587.1 hypothetical protein -
EJP81_RS24400 414002..414220 + 219 WP_013578112.1 YgdI/YgdR family lipoprotein -
EJP81_RS24405 414373..414639 + 267 WP_168444348.1 iron dicitrate ABC transporter ATP-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaRAHN-2 mgtB / vipA/tssB / vipB/tssC / hcp/tssD / clpV/tssH / hcp/tssD / hcp/tssD 1..556163 556163


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA-like, C-terminal

(166-381)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-139)


Sequences

Toxin        


Download         Length: 450 a.a.        Molecular weight: 51261.71 Da        Isoelectric Point: 4.7025

>T116915 WP_013578107.1 NZ_CP034838:c410106-408757 [Rahnella aquatilis]
MEFLTLQAFLNNTWVDIANITFPGSEQNNYRLTELNYHSDYAIDNLDRDDNHAASLNHPVSLFFDGDDERGWLKFLDDII
PSGSSRRYWANYLDLEGLTADQQNFILLKHGTLSPVGNLRIKESLPEYNPLAEKLFFTIEDVKNRAGDFLDYAQQRGAAA
GGATGAGGEAPKLLLRCSQEQQIWIDTYQNDTANRDIPYLVKYPRGARTEVDCNILRAEFYFYHELEAMGFDTIPTQNMR
LEEGLTYPSLWLPRFDISFEEEGLIRRFGMESVYSLLRKAPGVTLYHEETLRALIDKISHSHMVTEQGFDFDIPGFVTEW
VRRDLLNIIFGNSDNHGRNTSFLKDERSIRLAPIYDFAPMKADPEGIARTTKWKAPLEIGGEYDYPGIAQSLDDLVPAET
LLSALKNTSHQLSGLKERLEARGIPQQILNMPAISFSFIPEKLRRWGLL

Download         Length: 1350 bp

>T116915 NZ_CP034838:c410106-408757 [Rahnella aquatilis]
ATGGAATTTTTAACGTTACAAGCTTTCCTCAATAATACCTGGGTTGATATTGCTAACATCACTTTTCCCGGCAGTGAGCA
GAATAACTATCGTCTGACCGAACTGAATTATCATTCGGATTACGCTATTGATAACCTTGATCGTGACGATAACCATGCCG
CATCGTTGAATCACCCCGTCTCTTTGTTTTTTGATGGTGACGATGAGCGGGGTTGGCTGAAGTTTCTTGATGATATTATT
CCCAGCGGTTCAAGTCGCCGCTATTGGGCAAACTATCTCGATCTCGAAGGACTGACAGCCGATCAGCAAAATTTCATTTT
GCTCAAACATGGCACCTTGTCCCCTGTTGGCAATCTGCGCATAAAAGAATCATTACCGGAATATAATCCGCTGGCTGAAA
AACTTTTCTTCACTATAGAAGATGTTAAAAACCGGGCGGGTGACTTTCTGGACTACGCACAACAACGCGGTGCGGCTGCC
GGTGGCGCGACAGGCGCCGGGGGCGAAGCACCAAAGCTGTTGCTGCGTTGTAGCCAGGAACAGCAAATCTGGATTGATAC
CTACCAGAATGACACTGCCAACCGGGACATTCCCTATTTGGTCAAATATCCCCGTGGCGCAAGAACAGAGGTAGACTGCA
ATATTCTTCGGGCAGAATTCTATTTCTACCATGAGCTTGAAGCCATGGGGTTTGACACGATCCCGACGCAAAACATGCGG
CTGGAAGAAGGGCTGACTTATCCGTCACTCTGGCTTCCGCGTTTTGATATTTCTTTTGAAGAGGAAGGACTTATCAGACG
TTTCGGAATGGAATCAGTCTATTCACTCCTGCGCAAAGCGCCCGGTGTGACGCTGTATCATGAAGAAACCCTCCGTGCAC
TGATCGATAAGATTTCACACAGTCATATGGTGACAGAACAAGGTTTTGATTTTGATATCCCGGGGTTTGTGACTGAATGG
GTACGACGAGATCTGCTCAATATTATTTTTGGTAACAGCGATAATCATGGGCGTAACACCTCTTTCCTGAAAGATGAACG
CAGCATCCGGCTTGCACCAATCTACGATTTTGCGCCGATGAAAGCCGATCCGGAAGGTATTGCCAGAACGACGAAATGGA
AGGCTCCGTTGGAAATCGGCGGTGAATACGATTATCCCGGCATTGCACAATCACTCGATGATCTTGTCCCGGCAGAAACA
TTACTTTCGGCACTTAAAAACACGTCACATCAACTGTCGGGACTGAAAGAGCGGCTGGAAGCCCGTGGCATACCGCAGCA
AATTTTGAACATGCCCGCGATAAGTTTCAGTTTTATTCCTGAAAAGTTACGCCGCTGGGGGTTGTTATGA

Antitoxin


Download         Length: 158 a.a.        Molecular weight: 17587.18 Da        Isoelectric Point: 10.9653

>AT116915 WP_013578106.1 NZ_CP034838:c408760-408287 [Rahnella aquatilis]
MKEPEIAANSARNTIDKIRAKQNEANHVRRDAGQMLAQEETSSTGKKQAQRRVSNLTKPVSTNDRNQARLDIMRALLLGE
ITQGQALKALRITVLGLKQDAFGKLVTVSRKTLSEVENDKGNYTPDIINKLFRPFGLKVGLVPVSQQIFRTLFERSE

Download         Length: 474 bp

>AT116915 NZ_CP034838:c408760-408287 [Rahnella aquatilis]
ATGAAAGAACCAGAGATTGCCGCAAACTCTGCGAGAAATACCATTGATAAAATACGGGCTAAACAAAATGAGGCGAATCA
TGTGCGGCGTGACGCCGGACAAATGCTGGCTCAGGAAGAAACATCTTCCACGGGAAAAAAACAAGCCCAGCGAAGAGTGT
CGAACCTGACAAAACCCGTCAGCACCAATGACCGAAATCAAGCCCGGCTCGACATCATGCGGGCGTTACTTTTGGGTGAA
ATCACGCAGGGACAAGCACTAAAAGCACTGAGGATTACGGTGCTGGGGTTAAAACAAGACGCCTTTGGGAAACTAGTGAC
AGTGTCACGTAAGACGCTATCAGAAGTGGAAAATGATAAGGGAAATTACACGCCTGACATCATCAACAAACTGTTTAGAC
CATTTGGCCTGAAAGTCGGGCTGGTGCCGGTTTCTCAGCAGATATTCAGGACTCTCTTTGAGCGCAGTGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB H8P0A1

Antitoxin

Source ID Structure
AlphaFold DB H8P0A0

References