Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-couple_hipB
Location 364373..366192 Replicon plasmid pKM05
Accession NZ_CP034837
Organism Rahnella aquatilis strain KM05

Toxin (Protein)


Gene name hipA Uniprot ID -
Locus tag EJP80_RS24700 Protein ID WP_119262329.1
Coordinates 364843..366192 (-) Length 450 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID H8P0A0
Locus tag EJP80_RS24695 Protein ID WP_013578106.1
Coordinates 364373..364846 (-) Length 158 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EJP80_RS24670 360053..361552 + 1500 WP_013578103.1 UbiD family decarboxylase -
EJP80_RS24675 361612..362181 + 570 WP_121019887.1 UbiX family flavin prenyltransferase -
EJP80_RS24680 362221..362934 + 714 WP_112151437.1 DUF554 domain-containing protein -
EJP80_RS24685 363022..363876 + 855 WP_014416647.1 LysR family transcriptional regulator -
EJP80_RS24690 363992..364312 + 321 WP_013578105.1 multidrug efflux SMR transporter -
EJP80_RS24695 364373..364846 - 474 WP_013578106.1 helix-turn-helix domain-containing protein Antitoxin
EJP80_RS24700 364843..366192 - 1350 WP_119262329.1 type II toxin-antitoxin system HipA family toxin Toxin
EJP80_RS24705 366388..366609 - 222 WP_014416648.1 DUF1471 domain-containing protein -
EJP80_RS24710 366777..367400 - 624 WP_112151436.1 NAD(P)H-binding protein -
EJP80_RS24715 367397..367918 - 522 WP_041673402.1 heme utilization cystosolic carrier protein HutX -
EJP80_RS24720 367896..369221 - 1326 WP_014416651.1 heme anaerobic degradation radical SAM methyltransferase ChuW/HutW -
EJP80_RS24725 369334..369684 - 351 WP_112151587.1 hypothetical protein -
EJP80_RS24730 370087..370305 + 219 WP_013578112.1 YgdI/YgdR family lipoprotein -
EJP80_RS24735 370470..370763 + 294 Protein_368 iron dicitrate ABC transporter ATP-binding protein -
EJP80_RS24740 370859..371077 + 219 WP_014416654.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA-like, C-terminal

(166-381)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-139)


Sequences

Toxin        


Download         Length: 450 a.a.        Molecular weight: 51233.66 Da        Isoelectric Point: 4.6576

>T116913 WP_119262329.1 NZ_CP034837:c366192-364843 [Rahnella aquatilis]
MEFLTLQAFLNNTWVDIANITFPGSEQNNYRLTELNYHSDYAIDNLDRDDNHAASLNHPVSLFFDGDDERGWLKFLDDII
PSGSSRRYWANYLDLEGLTADQQNFILLKHGTLSPVGNLRIKESLPEYNPLAEKLFFTIEDVKNRAGDFLDYAQQRGAAA
GGATGAGGEAPKLLLRCSQEQQIWIDTYQNDTANRDIPYLVKYPRGARTEVDCNILRAEFYFYHELEAMGFDTIPTQNMR
LEEGLTYPSLWLPRFDISFEEEGLIRRFGMESVYSLLRKAPGVTLYHEETLRALIDKISHSHMVTEQGFDFDIPGFVTEW
VQRDLLNIIFGNSDNHGRNTSFLKDERSIRLAPIYDFAPMKADPEGIARTTKWKAPLEIGGEYDYPGIAQSLDDLVPAET
LLSALKNTSHQLSGLKERLEARGIPQQILNMPAISFSFIPEKLRRWGLL

Download         Length: 1350 bp

>T116913 NZ_CP034837:c366192-364843 [Rahnella aquatilis]
ATGGAATTTTTAACGTTACAAGCTTTCCTCAATAATACCTGGGTTGATATTGCTAACATCACTTTTCCCGGCAGTGAGCA
GAATAACTATCGTCTGACCGAACTGAATTATCATTCGGATTACGCTATTGATAACCTTGATCGTGACGATAACCATGCCG
CATCGTTGAATCACCCCGTCTCTTTGTTTTTTGATGGTGACGATGAGCGGGGTTGGCTGAAGTTTCTTGATGATATTATT
CCCAGCGGTTCAAGTCGCCGCTATTGGGCAAACTATCTCGATCTCGAAGGACTGACAGCCGATCAGCAAAATTTCATTTT
GCTCAAACATGGCACCTTGTCCCCTGTTGGCAATCTGCGCATAAAAGAATCATTACCGGAATATAATCCGCTGGCTGAAA
AACTTTTCTTCACTATAGAAGATGTTAAAAACCGGGCGGGTGACTTTCTGGACTACGCACAACAACGCGGTGCGGCTGCC
GGTGGCGCGACAGGCGCCGGGGGCGAAGCACCAAAGCTGTTGCTGCGTTGTAGTCAGGAACAGCAAATCTGGATTGATAC
CTACCAGAATGACACTGCCAACCGGGACATTCCCTATTTGGTCAAATATCCCCGTGGCGCAAGAACAGAGGTAGACTGCA
ATATTCTTCGGGCAGAATTCTATTTCTACCATGAGCTTGAAGCCATGGGGTTTGACACGATCCCGACGCAAAACATGCGG
CTGGAAGAAGGGCTGACTTATCCGTCACTCTGGCTTCCGCGTTTTGATATTTCTTTTGAAGAGGAAGGACTTATCAGACG
TTTCGGAATGGAATCAGTCTATTCACTCCTGCGCAAAGCGCCCGGTGTGACGCTGTATCATGAAGAAACCCTCCGTGCAC
TGATCGATAAGATTTCACACAGTCATATGGTGACAGAACAAGGTTTTGATTTTGATATCCCGGGGTTTGTGACTGAATGG
GTACAACGAGATCTGCTCAATATTATTTTTGGTAACAGCGATAATCATGGGCGTAACACCTCTTTCCTGAAAGATGAACG
CAGCATCCGGCTTGCACCAATCTACGATTTTGCGCCGATGAAAGCCGATCCGGAAGGTATTGCCAGAACGACGAAATGGA
AGGCTCCGCTGGAAATCGGCGGTGAATACGATTATCCCGGCATTGCACAATCACTCGATGATCTTGTCCCGGCAGAAACA
TTACTTTCGGCACTTAAAAACACGTCACATCAACTGTCGGGACTGAAAGAGCGGCTGGAAGCCCGTGGCATACCGCAGCA
AATTTTGAACATGCCCGCGATAAGTTTCAGTTTTATTCCTGAAAAGTTACGCCGCTGGGGGTTGTTATGA

Antitoxin


Download         Length: 158 a.a.        Molecular weight: 17587.18 Da        Isoelectric Point: 10.9653

>AT116913 WP_013578106.1 NZ_CP034837:c364846-364373 [Rahnella aquatilis]
MKEPEIAANSARNTIDKIRAKQNEANHVRRDAGQMLAQEETSSTGKKQAQRRVSNLTKPVSTNDRNQARLDIMRALLLGE
ITQGQALKALRITVLGLKQDAFGKLVTVSRKTLSEVENDKGNYTPDIINKLFRPFGLKVGLVPVSQQIFRTLFERSE

Download         Length: 474 bp

>AT116913 NZ_CP034837:c364846-364373 [Rahnella aquatilis]
ATGAAAGAACCAGAGATTGCCGCAAACTCTGCGAGAAATACCATTGATAAAATACGGGCTAAACAAAATGAGGCGAATCA
TGTGCGGCGTGACGCCGGACAAATGCTGGCTCAGGAAGAAACATCTTCCACGGGAAAAAAACAAGCCCAGCGAAGAGTGT
CGAACCTGACGAAACCCGTCAGCACCAATGACCGAAATCAAGCCCGGCTCGACATCATGCGGGCGTTACTTTTGGGTGAA
ATCACGCAGGGACAAGCACTAAAAGCACTGAGGATTACGGTGCTGGGGTTAAAACAAGACGCCTTTGGGAAATTAGTGAC
AGTGTCACGTAAGACGCTATCAGAAGTGGAAAATGATAAGGGAAATTACACGCCTGACATCATCAACAAACTGTTTAGAC
CATTTGGCCTGAAAGTCGGGCTGGTGCCGGTTTCTCAGCAGATATTCAGGACTCTCTTTGAGCGCAGTGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB H8P0A0

References