Detailed information of TA system TA116865

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 9450..9985
• Replicon: plasmid unnamed8
• Accession: NZ_CP034818
• Organism: Paracoccus sp. Arc7-R13

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: EOJ32_RS19920
• Protein ID: WP_127900037.1
• Coordinates: 9450..9698 (+)
• Length: 83 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: EOJ32_RS19925
• Protein ID: WP_127900038.1
• Coordinates: 9695..9985 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EOJ32_RS19880	4965..6062	-	1098	WP_127900031.1	MobA/MobL family protein	-
EOJ32_RS19885	6219..6470	+	252	WP_015062938.1	hypothetical protein	-
EOJ32_RS19890	6467..7027	+	561	WP_127900032.1	hypothetical protein	-
EOJ32_RS19895	7186..7365	+	180	WP_127900033.1	hypothetical protein	-
EOJ32_RS19900	7441..7659	+	219	WP_045983914.1	hypothetical protein	-
EOJ32_RS19905	7884..8090	+	207	WP_127900034.1	hypothetical protein	-
EOJ32_RS19910	8172..8486	-	315	WP_127900035.1	H-NS histone family protein	-
EOJ32_RS19915	8757..9050	+	294	WP_127900036.1	integration host factor subunit beta	-
EOJ32_RS19920	9450..9698	+	249	WP_127900037.1	BrnT family toxin	Toxin
EOJ32_RS19925	9695..9985	+	291	WP_127900038.1	BrnA antitoxin family protein	Antitoxin
EOJ32_RS19930	10424..10681	-	258	WP_127900039.1	hypothetical protein	-
EOJ32_RS19935	10965..11258	+	294	WP_127900040.1	integration host factor subunit beta	-
EOJ32_RS19940	11868..12410	+	543	WP_127900041.1	helix-turn-helix domain-containing protein	-
EOJ32_RS19945	12476..12907	+	432	WP_127900042.1	hypothetical protein	-
EOJ32_RS19950	13058..13395	+	338	Protein_20	hypothetical protein	-
EOJ32_RS19955	13921..14529	+	609	WP_127900043.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..24536	24536

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (6-78)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (20-94)

Sequences

Toxin

Protein

• Length: 83 a.a.
• Molecular weight: 8737.22 Da
• Isoelectric Point: 10.4637

>T116865 WP_127900037.1 NZ_CP034818:9450-9698 [Paracoccus sp. Arc7-R13]
MLMITWDAPKRQTNLAKHGLDFADLDEAFFLASVVVPAKAGRHMAIGRLADGTVAVVFAALGTQGVSVISMRPASARERS
LL

Nucleotide

• Length: 249 bp

>T116865 NZ_CP034818:9450-9698 [Paracoccus sp. Arc7-R13]
ATGTTGATGATCACCTGGGACGCGCCGAAGCGGCAGACCAACCTTGCCAAGCACGGCCTGGATTTTGCCGATCTCGACGA
GGCGTTTTTTCTGGCCTCGGTGGTGGTCCCGGCAAAGGCGGGCCGGCACATGGCGATCGGGCGTCTCGCGGATGGCACCG
TGGCGGTGGTCTTCGCGGCCTTGGGCACCCAAGGGGTGTCGGTGATCTCGATGCGCCCCGCCAGCGCCAGGGAACGGAGC
CTGCTATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10548.02 Da
• Isoelectric Point: 10.7279

>AT116865 WP_127900038.1 NZ_CP034818:9695-9985 [Paracoccus sp. Arc7-R13]
MSKTHLTEDQIQRMIASDPDAPEATAAQLTQARPFTEAFPALSDAMRRNMGGRPKVENPKVAVSLRLDQDVVARFKASGP
GWQTRMNRALREAAGL

Nucleotide

• Length: 291 bp

>AT116865 NZ_CP034818:9695-9985 [Paracoccus sp. Arc7-R13]
ATGAGCAAGACCCATCTGACCGAAGACCAGATCCAGCGGATGATCGCCAGTGATCCGGACGCCCCGGAGGCCACAGCGGC
GCAGCTCACTCAGGCCCGGCCCTTCACCGAGGCCTTCCCCGCCCTGTCCGACGCCATGCGCCGGAACATGGGCGGACGGC
CCAAGGTCGAAAACCCCAAGGTTGCCGTCTCGCTGCGCCTCGATCAGGACGTGGTGGCCCGGTTCAAGGCCAGCGGGCCG
GGCTGGCAGACCCGGATGAACCGTGCCCTGCGAGAGGCTGCCGGGCTCTGA