Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 81172..81697 Replicon plasmid p08-3914
Accession NZ_CP034809
Organism Escherichia coli strain 08-3914

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag AYP36_RS28760 Protein ID WP_001159868.1
Coordinates 81392..81697 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag AYP36_RS28755 Protein ID WP_000813634.1
Coordinates 81172..81390 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AYP36_RS28720 76455..76685 + 231 WP_010891288.1 hypothetical protein -
AYP36_RS28725 76806..77546 + 741 WP_001066920.1 tyrosine-type recombinase/integrase -
AYP36_RS28730 77831..78808 - 978 WP_000361615.1 RepB family plasmid replication initiator protein -
AYP36_RS28740 79216..79416 - 201 WP_000708307.1 hypothetical protein -
AYP36_RS28745 79413..80033 - 621 WP_001248529.1 hypothetical protein -
AYP36_RS28750 80030..80713 - 684 WP_010891291.1 DNA-binding protein -
AYP36_RS28755 81172..81390 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
AYP36_RS28760 81392..81697 + 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
AYP36_RS28765 81698..82504 + 807 WP_000016989.1 site-specific integrase -
AYP36_RS28780 85180..86346 + 1167 WP_000772446.1 plasmid-partitioning protein SopA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - toxB / espP / stcE / exeE / exeG / hlyC / hlyA / hlyB / hlyD 1..94980 94980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T116863 WP_001159868.1 NZ_CP034809:81392-81697 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T116863 NZ_CP034809:81392-81697 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT116863 WP_000813634.1 NZ_CP034809:81172-81390 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT116863 NZ_CP034809:81172-81390 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References