Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3033104..3033758 Replicon chromosome
Accession NZ_CP034808
Organism Escherichia coli strain 08-3914

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag AYP36_RS14655 Protein ID WP_000244781.1
Coordinates 3033351..3033758 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag AYP36_RS14650 Protein ID WP_000354046.1
Coordinates 3033104..3033370 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AYP36_RS14630 3029192..3030625 - 1434 WP_001310226.1 6-phospho-beta-glucosidase BglA -
AYP36_RS14635 3030670..3030981 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
AYP36_RS14640 3031145..3031804 + 660 WP_000250274.1 hemolysin III family protein -
AYP36_RS14645 3031881..3032861 - 981 WP_000886053.1 tRNA-modifying protein YgfZ -
AYP36_RS14650 3033104..3033370 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
AYP36_RS14655 3033351..3033758 + 408 WP_000244781.1 protein YgfX Toxin
AYP36_RS14660 3033798..3034319 - 522 WP_001055874.1 flavodoxin FldB -
AYP36_RS14665 3034431..3035327 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
AYP36_RS14670 3035352..3036062 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
AYP36_RS14675 3036068..3037801 + 1734 WP_000813185.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T116847 WP_000244781.1 NZ_CP034808:3033351-3033758 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T116847 NZ_CP034808:3033351-3033758 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT116847 WP_000354046.1 NZ_CP034808:3033104-3033370 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT116847 NZ_CP034808:3033104-3033370 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTATGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCCGATC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References