Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 2452243..2452874 Replicon chromosome
Accession NZ_CP034808
Organism Escherichia coli strain 08-3914

Toxin (Protein)


Gene name hicA Uniprot ID U9Y2A7
Locus tag AYP36_RS11625 Protein ID WP_001259385.1
Coordinates 2452243..2452518 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag AYP36_RS11630 Protein ID WP_000593555.1
Coordinates 2452515..2452874 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AYP36_RS11610 2447247..2448314 + 1068 WP_000361458.1 HlyD family secretion protein -
AYP36_RS11615 2448311..2451046 + 2736 WP_000149101.1 ribosome-associated ATPase/putative transporter RbbA -
AYP36_RS11620 2451046..2452170 + 1125 WP_001301659.1 ABC transporter permease -
AYP36_RS11625 2452243..2452518 + 276 WP_001259385.1 type II toxin-antitoxin system HicA family toxin Toxin
AYP36_RS11630 2452515..2452874 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
AYP36_RS11635 2452924..2453784 - 861 WP_001131788.1 class II aldolase -
AYP36_RS11640 2453816..2454085 - 270 WP_000933060.1 HPr family phosphocarrier protein -
AYP36_RS11645 2454075..2455583 - 1509 WP_001301773.1 carbohydrate kinase -
AYP36_RS11650 2455576..2456934 - 1359 WP_001302220.1 PTS galactitol transporter subunit IIC -
AYP36_RS11655 2457011..2457292 - 282 WP_000084025.1 PTS sugar transporter subunit IIB -
AYP36_RS11660 2457289..2457762 - 474 WP_001161553.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 2443387..2452874 9487


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10199.94 Da        Isoelectric Point: 10.7076

>T116841 WP_001259385.1 NZ_CP034808:2452243-2452518 [Escherichia coli]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T116841 NZ_CP034808:2452243-2452518 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT116841 WP_000593555.1 NZ_CP034808:2452515-2452874 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT116841 NZ_CP034808:2452515-2452874 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTW8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References