Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 60491..61016 Replicon plasmid p2010C-3347
Accession NZ_CP034807
Organism Escherichia coli strain 2010C-3347

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag A1D75_RS28820 Protein ID WP_001159868.1
Coordinates 60711..61016 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag A1D75_RS28815 Protein ID WP_000813634.1
Coordinates 60491..60709 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A1D75_RS28780 55774..56004 + 231 WP_010891288.1 hypothetical protein -
A1D75_RS28785 56125..56865 + 741 WP_001066920.1 tyrosine-type recombinase/integrase -
A1D75_RS28790 57150..58127 - 978 WP_000361615.1 RepB family plasmid replication initiator protein -
A1D75_RS28800 58535..58735 - 201 WP_000708307.1 hypothetical protein -
A1D75_RS28805 58732..59352 - 621 WP_001248529.1 hypothetical protein -
A1D75_RS28810 59349..60032 - 684 WP_010891291.1 DNA-binding protein -
A1D75_RS28815 60491..60709 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
A1D75_RS28820 60711..61016 + 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
A1D75_RS28835 63076..64242 + 1167 WP_000772446.1 plasmid-partitioning protein SopA -
A1D75_RS28840 64242..65213 + 972 WP_000817031.1 ParB/RepB/Spo0J family plasmid partition protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - toxB / espP / stcE / exeE / exeG / hlyC / hlyA / hlyB / hlyD / toxB / toxB 1..91176 91176


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T116827 WP_001159868.1 NZ_CP034807:60711-61016 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T116827 NZ_CP034807:60711-61016 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT116827 WP_000813634.1 NZ_CP034807:60491-60709 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT116827 NZ_CP034807:60491-60709 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References