Detailed information of TA system TA116792

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 81954..82513
• Replicon: plasmid p2009C-3554-2
• Accession: NZ_CP034805
• Organism: Escherichia coli strain 2009C-3554

Toxin (Protein)

• Gene name: relE
• Uniprot ID: O82928
• Locus tag: A0I22_RS29145
• Protein ID: WP_000421248.1
• Coordinates: 81954..82229 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: C1J8K5
• Locus tag: A0I22_RS29150
• Protein ID: WP_001178089.1
• Coordinates: 82229..82513 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A0I22_RS29110	77307..77696	-	390	WP_001172748.1	cytochrome b562 family protein	-
A0I22_RS29115	77740..79950	-	2211	WP_000592771.1	catalase/peroxidase KatP	-
A0I22_RS29120	80127..80312	+	186	WP_001302179.1	hypothetical protein	-
A0I22_RS29125	80312..80728	+	417	Protein_75	IS91 family transposase	-
A0I22_RS29130	80728..81294	+	567	Protein_76	IS3-like element IS600 family transposase	-
A0I22_RS29135	81338..81553	+	216	Protein_77	transposase	-
A0I22_RS29140	81653..81859	-	207	WP_001105064.1	hypothetical protein	-
A0I22_RS29145	81954..82229	-	276	WP_000421248.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
A0I22_RS29150	82229..82513	-	285	WP_001178089.1	ribbon-helix-helix domain-containing protein	Antitoxin
A0I22_RS29165	83425..84282	-	858	WP_000130945.1	incFII family plasmid replication initiator RepA	-
A0I22_RS29170	84275..84349	-	75	WP_001370046.1	RepA leader peptide Tap	-
A0I22_RS29180	84585..84839	-	255	WP_000083831.1	replication regulatory protein RepA	-
A0I22_RS29185	85079..85417	-	339	WP_000766796.1	hypothetical protein	-
A0I22_RS29190	85455..85664	-	210	WP_001302200.1	hemolysin expression modulator Hha	-
A0I22_RS29195	85710..86171	-	462	WP_001233853.1	thermonuclease family protein	-
A0I22_RS29200	86416..86628	-	213	WP_001302189.1	ANR family transcriptional regulator	-
A0I22_RS29205	86761..87321	-	561	WP_000840472.1	fertility inhibition protein FinO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	hlyD / hlyB / hlyA / hlyC / exeG / exeE / stcE / espP / toxB	1..94014	94014

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10735.42 Da
• Isoelectric Point: 9.9324

>T116792 WP_000421248.1 NZ_CP034805:c82229-81954 [Escherichia coli]
MELKWSSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T116792 NZ_CP034805:c82229-81954 [Escherichia coli]
ATGGAACTGAAGTGGAGCAGTAAAGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTAGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGGGAACAGTTATTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10474.89 Da
• Isoelectric Point: 5.6558

>AT116792 WP_001178089.1 NZ_CP034805:c82513-82229 [Escherichia coli]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT116792 NZ_CP034805:c82513-82229 [Escherichia coli]
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGTTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATTGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Structures

Toxin

• AlphaFold DB: A0A2Z6FAZ0

Antitoxin

• AlphaFold DB: A0A731IHA8