Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 34910..35435 Replicon plasmid p2009C-3554-2
Accession NZ_CP034805
Organism Escherichia coli strain 2009C-3554

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag A0I22_RS28885 Protein ID WP_001159868.1
Coordinates 34910..35215 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag A0I22_RS28890 Protein ID WP_000813634.1
Coordinates 35217..35435 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A0I22_RS28865 31867..32205 - 339 WP_071525396.1 RepB family plasmid replication initiator protein -
A0I22_RS28870 32167..33380 + 1214 WP_162829202.1 IS3 family transposase -
A0I22_RS28880 34103..34909 - 807 WP_000016989.1 site-specific integrase -
A0I22_RS28885 34910..35215 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
A0I22_RS28890 35217..35435 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
A0I22_RS28895 35894..36577 + 684 WP_010891291.1 DNA-binding protein -
A0I22_RS28900 36574..37194 + 621 WP_001248529.1 hypothetical protein -
A0I22_RS28905 37191..37391 + 201 WP_000708307.1 hypothetical protein -
A0I22_RS28915 37799..38776 + 978 WP_000361615.1 RepB family plasmid replication initiator protein -
A0I22_RS28920 39061..39801 - 741 WP_001066920.1 tyrosine-type recombinase/integrase -
A0I22_RS28925 39922..40152 - 231 WP_010891288.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - hlyD / hlyB / hlyA / hlyC / exeG / exeE / stcE / espP / toxB 1..94014 94014


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T116791 WP_001159868.1 NZ_CP034805:c35215-34910 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T116791 NZ_CP034805:c35215-34910 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT116791 WP_000813634.1 NZ_CP034805:c35435-35217 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT116791 NZ_CP034805:c35435-35217 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References