Detailed information of TA system TA116786

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 29235..29794
• Replicon: plasmid p2009C-3554-1
• Accession: NZ_CP034804
• Organism: Escherichia coli strain 2009C-3554

Toxin (Protein)

• Gene name: relE
• Uniprot ID: U9YB49
• Locus tag: A0I22_RS28295
• Protein ID: WP_000421259.1
• Coordinates: 29519..29794 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: U9Y625
• Locus tag: A0I22_RS28290
• Protein ID: WP_001178087.1
• Coordinates: 29235..29519 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A0I22_RS28240	24884..25417	-	534	WP_001372168.1	transcription termination factor NusG	-
A0I22_RS28250	25859..26146	-	288	WP_000074849.1	conjugal transfer protein TraA	-
A0I22_RS28255	26064..26327	-	264	WP_001324596.1	ash family protein	-
A0I22_RS28270	27300..28331	+	1032	WP_000907852.1	replication initiation protein	-
A0I22_RS28290	29235..29519	+	285	WP_001178087.1	ribbon-helix-helix domain-containing protein	Antitoxin
A0I22_RS28295	29519..29794	+	276	WP_000421259.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
A0I22_RS28300	29900..30181	+	282	WP_001105066.1	hypothetical protein	-
A0I22_RS29540	30373..30639	-	267	WP_000627832.1	hypothetical protein	-
A0I22_RS28310	30639..31133	-	495	WP_015058884.1	hypothetical protein	-
A0I22_RS28315	31189..31791	-	603	WP_000521606.1	hypothetical protein	-
A0I22_RS28320	31967..33019	-	1053	WP_000655519.1	hypothetical protein	-
A0I22_RS28325	33232..33822	-	591	WP_001401589.1	hypothetical protein	-
A0I22_RS28330	33822..34076	-	255	WP_000343106.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..83424	83424

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10736.45 Da
• Isoelectric Point: 9.9324

>T116786 WP_000421259.1 NZ_CP034804:29519-29794 [Escherichia coli]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELTGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T116786 NZ_CP034804:29519-29794 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
GGCCGCCAGAACGGTGCAGTCCCTGACACAGGCTCCGGTCATTCTGTTAACTCATCCACGTATGGGGGAACAGTTATTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTACTGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10488.92 Da
• Isoelectric Point: 5.6558

>AT116786 WP_001178087.1 NZ_CP034804:29235-29519 [Escherichia coli]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWIIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT116786 NZ_CP034804:29235-29519 [Escherichia coli]
ATGCAAATGAAAAACAATACCGCACAGGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGCTGGATTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Structures

Toxin

• AlphaFold DB: U9YB49

Antitoxin

• AlphaFold DB: U9Y625