Detailed information of TA system TA116768

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 2517380..2517975
• Replicon: chromosome
• Accession: NZ_CP034803
• Organism: Escherichia coli strain 2009C-3554

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: U9Y4M4
• Locus tag: A0I22_RS13165
• Protein ID: WP_000239579.1
• Coordinates: 2517380..2517730 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: Q8XCF3
• Locus tag: A0I22_RS13170
• Protein ID: WP_001223210.1
• Coordinates: 2517724..2517975 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A0I22_RS13145	2512826..2513848	-	1023	WP_001301928.1	ABC transporter permease	-
A0I22_RS13150	2513862..2515364	-	1503	WP_000205806.1	sugar ABC transporter ATP-binding protein	-
A0I22_RS13155	2515504..2516460	-	957	WP_000265942.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
A0I22_RS13160	2516770..2517300	+	531	WP_000055075.1	inorganic diphosphatase	-
A0I22_RS13165	2517380..2517730	-	351	WP_000239579.1	endoribonuclease toxin ChpB	Toxin
A0I22_RS13170	2517724..2517975	-	252	WP_001223210.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
A0I22_RS13175	2518187..2518528	-	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
A0I22_RS13180	2518531..2522310	-	3780	WP_000060930.1	autotransporter assembly complex protein TamB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12511.45 Da
• Isoelectric Point: 5.6219

>T116768 WP_000239579.1 NZ_CP034803:c2517730-2517380 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T116768 NZ_CP034803:c2517730-2517380 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GTGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTT
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9299.64 Da
• Isoelectric Point: 4.4201

>AT116768 WP_001223210.1 NZ_CP034803:c2517975-2517724 [Escherichia coli]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEVQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT116768 NZ_CP034803:c2517975-2517724 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGTGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y8A8