Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 424025..424663 Replicon chromosome
Accession NZ_CP034803
Organism Escherichia coli strain 2009C-3554

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag A0I22_RS02385 Protein ID WP_000813794.1
Coordinates 424487..424663 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag A0I22_RS02380 Protein ID WP_001270286.1
Coordinates 424025..424441 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A0I22_RS02360 419177..420118 - 942 WP_001251320.1 ABC transporter permease -
A0I22_RS02365 420119..421132 - 1014 WP_000220402.1 ABC transporter ATP-binding protein -
A0I22_RS02370 421150..422295 - 1146 WP_000047432.1 ABC transporter substrate-binding protein -
A0I22_RS02375 422540..423946 - 1407 WP_000760654.1 PLP-dependent aminotransferase family protein -
A0I22_RS02380 424025..424441 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
A0I22_RS02385 424487..424663 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
A0I22_RS02390 424885..425115 + 231 WP_000494244.1 YncJ family protein -
A0I22_RS02395 425207..427168 - 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
A0I22_RS02400 427241..427777 - 537 WP_000429145.1 DNA-binding transcriptional regulator SutR -
A0I22_RS02405 427830..429041 + 1212 WP_071525382.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T116758 WP_000813794.1 NZ_CP034803:c424663-424487 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T116758 NZ_CP034803:c424663-424487 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT116758 WP_001270286.1 NZ_CP034803:c424441-424025 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT116758 NZ_CP034803:c424441-424025 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAAGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCAGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References