Detailed information of TA system TA116752

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 39550..40109
• Replicon: plasmid p2010C-3142
• Accession: NZ_CP034802
• Organism: Escherichia coli strain 2010C-3142

Toxin (Protein)

• Gene name: relE
• Uniprot ID: O82928
• Locus tag: AYR10_RS28245
• Protein ID: WP_000421248.1
• Coordinates: 39834..40109 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: C1J8K5
• Locus tag: AYR10_RS28240
• Protein ID: WP_001178089.1
• Coordinates: 39550..39834 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AYR10_RS28185	34741..35301	+	561	WP_000840472.1	fertility inhibition protein FinO	-
AYR10_RS28190	35434..35646	+	213	WP_001302189.1	ANR family transcriptional regulator	-
AYR10_RS28195	35891..36352	+	462	WP_001233856.1	thermonuclease family protein	-
AYR10_RS28200	36398..36607	+	210	WP_001302200.1	hemolysin expression modulator Hha	-
AYR10_RS28205	36645..36983	+	339	WP_000766796.1	hypothetical protein	-
AYR10_RS28210	37223..37477	+	255	WP_000083831.1	replication regulatory protein RepA	-
AYR10_RS28220	37713..37787	+	75	WP_001370046.1	RepA leader peptide Tap	-
AYR10_RS28225	37780..38637	+	858	WP_000130945.1	incFII family plasmid replication initiator RepA	-
AYR10_RS28240	39550..39834	+	285	WP_001178089.1	ribbon-helix-helix domain-containing protein	Antitoxin
AYR10_RS28245	39834..40109	+	276	WP_000421248.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AYR10_RS28250	40204..40410	+	207	WP_001105064.1	hypothetical protein	-
AYR10_RS28255	40510..40725	-	216	Protein_47	transposase	-
AYR10_RS28260	40769..41335	-	567	Protein_48	IS3-like element IS600 family transposase	-
AYR10_RS28265	41335..41751	-	417	Protein_49	IS91 family transposase	-
AYR10_RS28270	41751..41936	-	186	WP_001302179.1	hypothetical protein	-
AYR10_RS28275	42113..44323	+	2211	WP_000592771.1	catalase/peroxidase KatP	-
AYR10_RS28280	44367..44756	+	390	WP_001172748.1	cytochrome b562 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	toxB / espP / stcE / exeE / exeG / hlyC / hlyA / hlyB / hlyD	1..94586	94586

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10735.42 Da
• Isoelectric Point: 9.9324

>T116752 WP_000421248.1 NZ_CP034802:39834-40109 [Escherichia coli]
MELKWSSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T116752 NZ_CP034802:39834-40109 [Escherichia coli]
ATGGAACTGAAGTGGAGCAGTAAAGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTAGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGGGAACAGTTATTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10474.89 Da
• Isoelectric Point: 5.6558

>AT116752 WP_001178089.1 NZ_CP034802:39550-39834 [Escherichia coli]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT116752 NZ_CP034802:39550-39834 [Escherichia coli]
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGTTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATTGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Structures

Toxin

• AlphaFold DB: A0A2Z6FAZ0

Antitoxin

• AlphaFold DB: A0A731IHA8