Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 4923573..4924211 Replicon chromosome
Accession NZ_CP034801
Organism Escherichia coli strain 2010C-3142

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag AYR10_RS24745 Protein ID WP_000813794.1
Coordinates 4924035..4924211 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag AYR10_RS24740 Protein ID WP_001270286.1
Coordinates 4923573..4923989 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AYR10_RS24720 4918725..4919666 - 942 WP_001251315.1 ABC transporter permease -
AYR10_RS24725 4919667..4920680 - 1014 WP_000220402.1 ABC transporter ATP-binding protein -
AYR10_RS24730 4920698..4921843 - 1146 WP_000047432.1 ABC transporter substrate-binding protein -
AYR10_RS24735 4922088..4923494 - 1407 WP_000760654.1 PLP-dependent aminotransferase family protein -
AYR10_RS24740 4923573..4923989 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
AYR10_RS24745 4924035..4924211 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
AYR10_RS24750 4924433..4924663 + 231 WP_000494244.1 YncJ family protein -
AYR10_RS24755 4924755..4926716 - 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
AYR10_RS24760 4926789..4927325 - 537 WP_000429145.1 DNA-binding transcriptional regulator SutR -
AYR10_RS24765 4927417..4928590 + 1174 Protein_4704 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T116750 WP_000813794.1 NZ_CP034801:c4924211-4924035 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T116750 NZ_CP034801:c4924211-4924035 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT116750 WP_001270286.1 NZ_CP034801:c4923989-4923573 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT116750 NZ_CP034801:c4923989-4923573 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAAGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References