Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 4708164..4708635 Replicon chromosome
Accession NZ_CP034801
Organism Escherichia coli strain 2010C-3142

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A0D7C2L1
Locus tag AYR10_RS23555 Protein ID WP_001303511.1
Coordinates 4708164..4708442 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag AYR10_RS23560 Protein ID WP_001302048.1
Coordinates 4708444..4708635 (-) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AYR10_RS23525 4705099..4706184 - 1086 Protein_4469 exonuclease -
AYR10_RS23530 4706277..4706468 - 192 WP_001090200.1 DUF1482 family protein -
AYR10_RS23535 4706465..4706653 - 189 WP_000449192.1 cell division inhibition protein DicB -
AYR10_RS23545 4707222..4707440 - 219 WP_001171930.1 hypothetical protein -
AYR10_RS23550 4707512..4707811 - 300 WP_001240334.1 hypothetical protein -
AYR10_RS23555 4708164..4708442 - 279 WP_001303511.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
AYR10_RS23560 4708444..4708635 - 192 WP_001302048.1 hypothetical protein Antitoxin
AYR10_RS23565 4708656..4709027 - 372 WP_001169686.1 hypothetical protein -
AYR10_RS23570 4709125..4709427 + 303 WP_000172738.1 transcriptional regulator -
AYR10_RS23575 4709424..4709849 + 426 WP_000693943.1 Rha family transcriptional regulator -
AYR10_RS23580 4709872..4710834 + 963 WP_000095669.1 helix-turn-helix domain-containing protein -
AYR10_RS23585 4710841..4711581 + 741 WP_000788938.1 ATP-binding protein -
AYR10_RS23590 4711607..4712376 + 770 Protein_4481 DUF1627 domain-containing protein -
AYR10_RS23595 4712392..4712787 + 396 WP_001118159.1 DUF977 family protein -
AYR10_RS23600 4712844..4713428 + 585 WP_000206793.1 DUF551 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - nleG7' 4698564..4799251 100687


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10661.25 Da        Isoelectric Point: 5.5647

>T116747 WP_001303511.1 NZ_CP034801:c4708442-4708164 [Escherichia coli]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T116747 NZ_CP034801:c4708442-4708164 [Escherichia coli]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT116747 WP_001302048.1 NZ_CP034801:c4708635-4708444 [Escherichia coli]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT116747 NZ_CP034801:c4708635-4708444 [Escherichia coli]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CZF
PDB 5CW7
PDB 5CZE

Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References