Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 4716243..4716714 Replicon chromosome
Accession NZ_CP034799
Organism Escherichia coli strain 2009C-4687

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A0D7C2L1
Locus tag A0I37_RS23625 Protein ID WP_001303511.1
Coordinates 4716243..4716521 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag A0I37_RS23630 Protein ID WP_001302048.1
Coordinates 4716523..4716714 (-) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A0I37_RS23595 4713178..4714263 - 1086 Protein_4479 exonuclease -
A0I37_RS23600 4714356..4714547 - 192 WP_001090200.1 DUF1482 family protein -
A0I37_RS23605 4714544..4714732 - 189 WP_000449192.1 cell division inhibition protein DicB -
A0I37_RS23615 4715301..4715519 - 219 WP_001171930.1 hypothetical protein -
A0I37_RS23620 4715591..4715890 - 300 WP_001240334.1 hypothetical protein -
A0I37_RS23625 4716243..4716521 - 279 WP_001303511.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
A0I37_RS23630 4716523..4716714 - 192 WP_001302048.1 hypothetical protein Antitoxin
A0I37_RS23635 4716735..4717106 - 372 WP_001169686.1 hypothetical protein -
A0I37_RS23640 4717204..4717506 + 303 WP_000172738.1 transcriptional regulator -
A0I37_RS23645 4717503..4717928 + 426 WP_000693943.1 Rha family transcriptional regulator -
A0I37_RS23650 4717951..4718913 + 963 WP_000095669.1 helix-turn-helix domain-containing protein -
A0I37_RS23655 4718920..4719660 + 741 WP_000788938.1 ATP-binding protein -
A0I37_RS23660 4719686..4720455 + 770 Protein_4491 DUF1627 domain-containing protein -
A0I37_RS23665 4720471..4720866 + 396 WP_001118159.1 DUF977 family protein -
A0I37_RS23670 4720923..4721507 + 585 WP_000206793.1 DUF551 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - nleG7' 4694922..4816796 121874


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10661.25 Da        Isoelectric Point: 5.5647

>T116712 WP_001303511.1 NZ_CP034799:c4716521-4716243 [Escherichia coli]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T116712 NZ_CP034799:c4716521-4716243 [Escherichia coli]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT116712 WP_001302048.1 NZ_CP034799:c4716714-4716523 [Escherichia coli]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT116712 NZ_CP034799:c4716714-4716523 [Escherichia coli]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CZF
PDB 5CW7
PDB 5CZE

Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References