Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3033835..3034489 Replicon chromosome
Accession NZ_CP034799
Organism Escherichia coli strain 2009C-4687

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag A0I37_RS14685 Protein ID WP_000244781.1
Coordinates 3034082..3034489 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag A0I37_RS14680 Protein ID WP_000354046.1
Coordinates 3033835..3034101 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A0I37_RS14660 3029923..3031356 - 1434 WP_001310226.1 6-phospho-beta-glucosidase BglA -
A0I37_RS14665 3031401..3031712 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
A0I37_RS14670 3031876..3032535 + 660 WP_000250274.1 hemolysin III family protein -
A0I37_RS14675 3032612..3033592 - 981 WP_000886053.1 tRNA-modifying protein YgfZ -
A0I37_RS14680 3033835..3034101 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
A0I37_RS14685 3034082..3034489 + 408 WP_000244781.1 protein YgfX Toxin
A0I37_RS14690 3034529..3035050 - 522 WP_001055874.1 flavodoxin FldB -
A0I37_RS14695 3035162..3036058 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
A0I37_RS14700 3036083..3036793 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
A0I37_RS14705 3036799..3038532 + 1734 WP_000813185.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T116702 WP_000244781.1 NZ_CP034799:3034082-3034489 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T116702 NZ_CP034799:3034082-3034489 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT116702 WP_000354046.1 NZ_CP034799:3033835-3034101 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT116702 NZ_CP034799:3033835-3034101 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTATGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCCGATC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References