Detailed information of TA system TA116682

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsxAB/Xre(antitoxin)
• Location: 6132..6633
• Replicon: plasmid p08-3918-2
• Accession: NZ_CP034798
• Organism: Escherichia coli strain 08-3918

Toxin (Protein)

• Gene name: TsxA
• Uniprot ID: A0A6X6XTU7
• Locus tag: AYP37_RS28115
• Protein ID: WP_000702245.1
• Coordinates: 6373..6633 (-)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: TsxB
• Uniprot ID: -
• Locus tag: AYP37_RS28110
• Protein ID: WP_001132407.1
• Coordinates: 6132..6383 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AYP37_RS28055	1138..1845	+	708	WP_000108795.1	hypothetical protein	-
AYP37_RS28060	1865..2311	+	447	WP_021580490.1	hypothetical protein	-
AYP37_RS28065	2314..2805	+	492	WP_021529587.1	hypothetical protein	-
AYP37_RS28070	2807..3070	+	264	WP_000224227.1	hypothetical protein	-
AYP37_RS28075	3081..3401	+	321	WP_000206710.1	hypothetical protein	-
AYP37_RS28080	3532..3969	-	438	WP_000466318.1	type II toxin-antitoxin system HicB family antitoxin	-
AYP37_RS28085	3994..4176	-	183	WP_000833470.1	type II toxin-antitoxin system HicA family toxin	-
AYP37_RS28090	4734..4916	+	183	WP_001401700.1	hypothetical protein	-
AYP37_RS28095	5304..5582	-	279	WP_000545938.1	hypothetical protein	-
AYP37_RS28110	6132..6383	-	252	WP_001132407.1	transcriptional regulator	Antitoxin
AYP37_RS28115	6373..6633	-	261	WP_000702245.1	hypothetical protein	Toxin
AYP37_RS28120	6685..6909	-	225	WP_000814384.1	hypothetical protein	-
AYP37_RS28125	7007..7216	-	210	WP_000866037.1	hypothetical protein	-
AYP37_RS28130	7257..7580	-	324	WP_000432876.1	hypothetical protein	-
AYP37_RS28140	8161..8490	-	330	WP_000855121.1	hypothetical protein	-
AYP37_RS28150	9088..9753	+	666	WP_000532148.1	P-loop NTPase	-
AYP37_RS28675	9827..10111	+	285	WP_001162371.1	hypothetical protein	-
AYP37_RS28160	10129..10725	-	597	WP_000125164.1	hypothetical protein	-
AYP37_RS28165	10875..11036	-	162	WP_001434039.1	type I toxin-antitoxin system Hok family toxin	-
AYP37_RS28515	11230..11391	-	162	WP_001128311.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..42137	42137

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (8-37)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 9818.23 Da
• Isoelectric Point: 9.5163

>T116682 WP_000702245.1 NZ_CP034798:c6633-6373 [Escherichia coli]
MKIRKGDRQYYLNKEGDTFHLVKRVKTFSKSATLGKTKATVKTVADLVFHEEAFDTIDFASDGLRENDKEIVSMMIQEMS
EGKNAK

Nucleotide

• Length: 261 bp

>T116682 NZ_CP034798:c6633-6373 [Escherichia coli]
ATGAAGATAAGAAAAGGTGACAGACAATATTATCTGAATAAAGAAGGTGATACGTTTCATCTGGTGAAGCGAGTAAAAAC
TTTTTCTAAGTCTGCGACTTTAGGAAAAACGAAAGCAACGGTGAAAACTGTTGCTGATCTTGTTTTTCATGAGGAGGCAT
TCGACACTATCGACTTTGCCAGCGACGGATTAAGAGAGAATGATAAAGAAATTGTTTCTATGATGATTCAGGAAATGAGT
GAGGGAAAAAATGCCAAATGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9404.77 Da
• Isoelectric Point: 9.8120

>AT116682 WP_001132407.1 NZ_CP034798:c6383-6132 [Escherichia coli]
MPNENTPENIKRLRQKIGLTQKECAEIFSMSPRTWRRKEEPVGTASGTALTPVEFKFLLLLAGEHPDYVLCKRNKKNSDS
GSN

Nucleotide

• Length: 252 bp

>AT116682 NZ_CP034798:c6383-6132 [Escherichia coli]
ATGCCAAATGAAAACACACCAGAAAACATTAAACGTCTGCGGCAAAAAATTGGCCTGACACAGAAAGAATGTGCTGAAAT
ATTCAGCATGAGTCCGCGTACCTGGCGTCGCAAAGAAGAACCTGTCGGAACTGCAAGCGGTACAGCACTTACACCAGTAG
AGTTTAAATTTCTTCTGCTTTTAGCTGGAGAACACCCTGATTATGTACTTTGTAAAAGGAATAAAAAGAATTCTGATTCT
GGAAGTAATTAA

Structures

Toxin

• AlphaFold DB: A0A6X6XTU7