Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4274530..4275148 Replicon chromosome
Accession NZ_CP034797
Organism Escherichia coli strain 08-3918

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag AYP37_RS22010 Protein ID WP_001291435.1
Coordinates 4274530..4274748 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag AYP37_RS22015 Protein ID WP_000344800.1
Coordinates 4274774..4275148 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AYP37_RS21975 4269817..4270389 + 573 WP_000779831.1 YbaY family lipoprotein -
AYP37_RS21980 4270422..4270733 - 312 WP_000409911.1 MGMT family protein -
AYP37_RS21990 4271112..4271465 + 354 WP_000878140.1 DUF1428 domain-containing protein -
AYP37_RS21995 4271507..4273057 - 1551 WP_001301851.1 cyclic-guanylate-specific phosphodiesterase PdeB -
AYP37_RS22000 4273221..4273691 - 471 WP_000136192.1 YlaC family protein -
AYP37_RS22005 4273807..4274358 - 552 WP_000102553.1 maltose O-acetyltransferase -
AYP37_RS22010 4274530..4274748 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
AYP37_RS22015 4274774..4275148 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
AYP37_RS22020 4275694..4278843 - 3150 WP_001132452.1 multidrug efflux RND transporter permease subunit -
AYP37_RS22025 4278866..4280059 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T116674 WP_001291435.1 NZ_CP034797:c4274748-4274530 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T116674 NZ_CP034797:c4274748-4274530 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT116674 WP_000344800.1 NZ_CP034797:c4275148-4274774 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT116674 NZ_CP034797:c4275148-4274774 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACTCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References