Detailed information of TA system TA116666

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 3530024..3530619
• Replicon: chromosome
• Accession: NZ_CP034797
• Organism: Escherichia coli strain 08-3918

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: U9Y4M4
• Locus tag: AYP37_RS18610
• Protein ID: WP_000239579.1
• Coordinates: 3530269..3530619 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: Q8XCF3
• Locus tag: AYP37_RS18605
• Protein ID: WP_001223210.1
• Coordinates: 3530024..3530275 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AYP37_RS18595	3525689..3529468	+	3780	WP_000060931.1	autotransporter assembly complex protein TamB	-
AYP37_RS18600	3529471..3529812	+	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
AYP37_RS18605	3530024..3530275	+	252	WP_001223210.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
AYP37_RS18610	3530269..3530619	+	351	WP_000239579.1	endoribonuclease toxin ChpB	Toxin
AYP37_RS18615	3530699..3531229	-	531	WP_000055075.1	inorganic diphosphatase	-
AYP37_RS18620	3531539..3532495	+	957	WP_000265942.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
AYP37_RS18625	3532635..3534137	+	1503	Protein_3429	sugar ABC transporter ATP-binding protein	-
AYP37_RS18630	3534151..3535173	+	1023	WP_001301928.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12511.45 Da
• Isoelectric Point: 5.6219

>T116666 WP_000239579.1 NZ_CP034797:3530269-3530619 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T116666 NZ_CP034797:3530269-3530619 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GTGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTT
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9299.64 Da
• Isoelectric Point: 4.4201

>AT116666 WP_001223210.1 NZ_CP034797:3530024-3530275 [Escherichia coli]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEVQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT116666 NZ_CP034797:3530024-3530275 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGTGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y8A8