Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 33185..33710 Replicon plasmid p08-3918-1
Accession NZ_CP034796
Organism Escherichia coli strain 08-3918

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag AYP37_RS00205 Protein ID WP_001159868.1
Coordinates 33185..33490 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag AYP37_RS00210 Protein ID WP_000813634.1
Coordinates 33492..33710 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AYP37_RS00180 28272..29438 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
AYP37_RS00185 30026..30388 - 363 Protein_31 RepB family plasmid replication initiator protein -
AYP37_RS00190 30442..31655 + 1214 WP_162829202.1 IS3 family transposase -
AYP37_RS00200 32378..33184 - 807 WP_000016989.1 site-specific integrase -
AYP37_RS00205 33185..33490 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
AYP37_RS00210 33492..33710 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
AYP37_RS00215 34169..34852 + 684 WP_010891291.1 DNA-binding protein -
AYP37_RS00220 34849..35469 + 621 WP_001248529.1 hypothetical protein -
AYP37_RS00225 35466..35666 + 201 WP_000708307.1 hypothetical protein -
AYP37_RS00235 36074..37051 + 978 WP_000361615.1 RepB family plasmid replication initiator protein -
AYP37_RS00240 37336..38076 - 741 WP_001066920.1 tyrosine-type recombinase/integrase -
AYP37_RS00245 38197..38427 - 231 WP_010891288.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - hlyD / hlyB / hlyA / hlyC / exeG / exeE / stcE / espP / toxB 1..94189 94189


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T116641 WP_001159868.1 NZ_CP034796:c33490-33185 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T116641 NZ_CP034796:c33490-33185 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT116641 WP_000813634.1 NZ_CP034796:c33710-33492 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT116641 NZ_CP034796:c33710-33492 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References