Detailed information of TA system TA116639

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 34358..34917
• Replicon: plasmid p06-3462
• Accession: NZ_CP034795
• Organism: Escherichia coli strain 06-3462

Toxin (Protein)

• Gene name: relE
• Uniprot ID: O82928
• Locus tag: A1D74_RS27915
• Protein ID: WP_000421248.1
• Coordinates: 34642..34917 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: C1J8K5
• Locus tag: A1D74_RS27910
• Protein ID: WP_001178089.1
• Coordinates: 34358..34642 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A1D74_RS27855	29550..30110	+	561	WP_000840472.1	fertility inhibition protein FinO	-
A1D74_RS27860	30243..30455	+	213	WP_001302189.1	ANR family transcriptional regulator	-
A1D74_RS27865	30700..31161	+	462	WP_001233856.1	thermonuclease family protein	-
A1D74_RS27870	31207..31416	+	210	WP_001302200.1	hemolysin expression modulator Hha	-
A1D74_RS27875	31454..31792	+	339	WP_000766796.1	hypothetical protein	-
A1D74_RS27880	32032..32286	+	255	WP_000083831.1	replication regulatory protein RepA	-
A1D74_RS27890	32522..32596	+	75	WP_001370046.1	RepA leader peptide Tap	-
A1D74_RS27895	32589..33446	+	858	WP_000130945.1	incFII family plasmid replication initiator RepA	-
A1D74_RS27910	34358..34642	+	285	WP_001178089.1	ribbon-helix-helix domain-containing protein	Antitoxin
A1D74_RS27915	34642..34917	+	276	WP_000421248.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
A1D74_RS27920	35012..35218	+	207	WP_001105064.1	hypothetical protein	-
A1D74_RS27925	35318..35533	-	216	Protein_41	transposase	-
A1D74_RS27930	35577..36143	-	567	Protein_42	IS3-like element IS600 family transposase	-
A1D74_RS27935	36143..36559	-	417	Protein_43	IS91 family transposase	-
A1D74_RS27940	36559..36744	-	186	WP_001302179.1	hypothetical protein	-
A1D74_RS27945	36921..39131	+	2211	WP_000592771.1	catalase/peroxidase KatP	-
A1D74_RS27950	39175..39564	+	390	WP_001172748.1	cytochrome b562 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	toxB / espP / stcE / exeE / exeG / hlyC / hlyA / hlyB / hlyD	1..94987	94987

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10735.42 Da
• Isoelectric Point: 9.9324

>T116639 WP_000421248.1 NZ_CP034795:34642-34917 [Escherichia coli]
MELKWSSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T116639 NZ_CP034795:34642-34917 [Escherichia coli]
ATGGAACTGAAGTGGAGCAGTAAAGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTAGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGGGAACAGTTATTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10474.89 Da
• Isoelectric Point: 5.6558

>AT116639 WP_001178089.1 NZ_CP034795:34358-34642 [Escherichia coli]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT116639 NZ_CP034795:34358-34642 [Escherichia coli]
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGTTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATTGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Structures

Toxin

• AlphaFold DB: A0A2Z6FAZ0

Antitoxin

• AlphaFold DB: A0A731IHA8