Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 1888023..1888494 Replicon chromosome
Accession NZ_CP034794
Organism Escherichia coli strain 06-3462

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A0D7C2L1
Locus tag A1D74_RS09760 Protein ID WP_001303511.1
Coordinates 1888216..1888494 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag A1D74_RS09755 Protein ID WP_001302048.1
Coordinates 1888023..1888214 (+) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A1D74_RS09725 1883764..1884504 - 741 WP_000788939.1 ATP-binding protein -
A1D74_RS09730 1884511..1885473 - 963 WP_127824039.1 helix-turn-helix domain-containing protein -
A1D74_RS09735 1885496..1885921 - 426 WP_000693943.1 Rha family transcriptional regulator -
A1D74_RS09740 1885918..1886151 - 234 Protein_1833 transcriptional regulator -
A1D74_RS09750 1887631..1888002 + 372 WP_001169686.1 hypothetical protein -
A1D74_RS09755 1888023..1888214 + 192 WP_001302048.1 hypothetical protein Antitoxin
A1D74_RS09760 1888216..1888494 + 279 WP_001303511.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
A1D74_RS09765 1888846..1889145 + 300 WP_001240334.1 hypothetical protein -
A1D74_RS09770 1889217..1889435 + 219 WP_001171930.1 hypothetical protein -
A1D74_RS09780 1890004..1890192 + 189 WP_000449192.1 cell division inhibition protein DicB -
A1D74_RS09785 1890189..1890380 + 192 WP_001090200.1 DUF1482 family protein -
A1D74_RS09790 1890473..1892944 + 2472 WP_000048458.1 exonuclease -
A1D74_RS09795 1893017..1893268 + 252 WP_000005552.1 excisionase family protein -
A1D74_RS09800 1893303..1893487 + 185 Protein_1844 DUF3596 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1845225..1896780 51555


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10661.25 Da        Isoelectric Point: 5.5647

>T116613 WP_001303511.1 NZ_CP034794:1888216-1888494 [Escherichia coli]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T116613 NZ_CP034794:1888216-1888494 [Escherichia coli]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT116613 WP_001302048.1 NZ_CP034794:1888023-1888214 [Escherichia coli]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT116613 NZ_CP034794:1888023-1888214 [Escherichia coli]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CZF
PDB 5CW7
PDB 5CZE

Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References