Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 1713358..1713996 Replicon chromosome
Accession NZ_CP034794
Organism Escherichia coli strain 06-3462

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag A1D74_RS08870 Protein ID WP_000813794.1
Coordinates 1713358..1713534 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag A1D74_RS08875 Protein ID WP_001270286.1
Coordinates 1713580..1713996 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A1D74_RS08850 1708979..1710152 - 1174 Protein_1663 BenE family transporter YdcO -
A1D74_RS08855 1710244..1710780 + 537 WP_000429145.1 DNA-binding transcriptional regulator SutR -
A1D74_RS08860 1710853..1712814 + 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
A1D74_RS08865 1712906..1713136 - 231 WP_000494244.1 YncJ family protein -
A1D74_RS08870 1713358..1713534 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
A1D74_RS08875 1713580..1713996 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
A1D74_RS08880 1714075..1715481 + 1407 WP_000760654.1 PLP-dependent aminotransferase family protein -
A1D74_RS08885 1715726..1716871 + 1146 WP_000047432.1 ABC transporter substrate-binding protein -
A1D74_RS08890 1716889..1717902 + 1014 WP_000220402.1 ABC transporter ATP-binding protein -
A1D74_RS08895 1717903..1718844 + 942 WP_001251315.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T116612 WP_000813794.1 NZ_CP034794:1713358-1713534 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T116612 NZ_CP034794:1713358-1713534 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT116612 WP_001270286.1 NZ_CP034794:1713580-1713996 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT116612 NZ_CP034794:1713580-1713996 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAAGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References