Detailed information of TA system TA116603

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 55249..55808
• Replicon: plasmid p2009C-3378
• Accession: NZ_CP034793
• Organism: Escherichia coli strain 2009C-3378

Toxin (Protein)

• Gene name: relE
• Uniprot ID: O82928
• Locus tag: DR788_RS27870
• Protein ID: WP_000421248.1
• Coordinates: 55249..55524 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: C1J8K5
• Locus tag: DR788_RS27875
• Protein ID: WP_001178089.1
• Coordinates: 55524..55808 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DR788_RS27835	50602..50991	-	390	WP_001172748.1	cytochrome b562 family protein	-
DR788_RS27840	51035..53245	-	2211	WP_000592771.1	catalase/peroxidase KatP	-
DR788_RS27845	53422..53607	+	186	WP_001302179.1	hypothetical protein	-
DR788_RS27850	53607..54023	+	417	Protein_48	IS91 family transposase	-
DR788_RS27855	54023..54589	+	567	Protein_49	IS3-like element IS600 family transposase	-
DR788_RS27860	54633..54848	+	216	Protein_50	transposase	-
DR788_RS27865	54948..55154	-	207	WP_001105064.1	hypothetical protein	-
DR788_RS27870	55249..55524	-	276	WP_000421248.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DR788_RS27875	55524..55808	-	285	WP_001178089.1	ribbon-helix-helix domain-containing protein	Antitoxin
DR788_RS27890	56721..57578	-	858	WP_000130945.1	incFII family plasmid replication initiator RepA	-
DR788_RS27895	57571..57645	-	75	WP_001370046.1	RepA leader peptide Tap	-
DR788_RS27905	57881..58135	-	255	WP_000083831.1	replication regulatory protein RepA	-
DR788_RS27910	58375..58713	-	339	WP_000766796.1	hypothetical protein	-
DR788_RS27915	58751..58960	-	210	WP_001302200.1	hemolysin expression modulator Hha	-
DR788_RS27920	59006..59467	-	462	WP_001233856.1	thermonuclease family protein	-
DR788_RS27925	59712..59924	-	213	WP_001302189.1	ANR family transcriptional regulator	-
DR788_RS27930	60057..60617	-	561	WP_000840472.1	fertility inhibition protein FinO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	hlyD / hlyB / hlyA / hlyC / exeG / exeE / stcE / espP / toxB	1..95421	95421

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10735.42 Da
• Isoelectric Point: 9.9324

>T116603 WP_000421248.1 NZ_CP034793:c55524-55249 [Escherichia coli]
MELKWSSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T116603 NZ_CP034793:c55524-55249 [Escherichia coli]
ATGGAACTGAAGTGGAGCAGTAAAGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTAGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGGGAACAGTTATTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10474.89 Da
• Isoelectric Point: 5.6558

>AT116603 WP_001178089.1 NZ_CP034793:c55808-55524 [Escherichia coli]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT116603 NZ_CP034793:c55808-55524 [Escherichia coli]
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGTTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATTGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Structures

Toxin

• AlphaFold DB: A0A2Z6FAZ0

Antitoxin

• AlphaFold DB: A0A731IHA8