Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 146273..146874 Replicon plasmid pTB422
Accession NZ_CP034789
Organism Escherichia coli strain ECCNB20-2

Toxin (Protein)


Gene name doc Uniprot ID U9YA20
Locus tag CRG85_RS25515 Protein ID WP_001216045.1
Coordinates 146273..146653 (-) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag CRG85_RS25520 Protein ID WP_001190712.1
Coordinates 146653..146874 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CRG85_RS25470 141577..142274 - 698 Protein_145 IS1 family transposase -
CRG85_RS25480 142445..142654 + 210 WP_000874156.1 hypothetical protein -
CRG85_RS25485 142765..143616 + 852 WP_000611664.1 hypothetical protein -
CRG85_RS25490 143649..143867 - 219 Protein_148 phage antirepressor KilAC domain-containing protein -
CRG85_RS25500 143926..144894 + 969 WP_000654811.1 IS5 family transposase -
CRG85_RS25505 145018..146061 - 1044 WP_072647226.1 DUF968 domain-containing protein -
CRG85_RS25510 146089..146268 - 180 WP_000113018.1 hypothetical protein -
CRG85_RS25515 146273..146653 - 381 WP_001216045.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
CRG85_RS25520 146653..146874 - 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
CRG85_RS25525 147057..148613 + 1557 WP_072647227.1 type I restriction-modification system subunit M -
CRG85_RS25530 148610..149878 + 1269 WP_072647224.1 restriction endonuclease subunit S -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid mph(A) / oqxA / oqxB / sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) / blaTEM-1B / sul1 / qacE / ant(3'')-Ia / dfrA1 - 1..159108 159108


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(3-120)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13588.29 Da        Isoelectric Point: 5.1514

>T116568 WP_001216045.1 NZ_CP034789:c146653-146273 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T116568 NZ_CP034789:c146653-146273 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTGATAGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCGGGAATGTC
AGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGTTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGGGCTGCGACCGG
AGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCCGCGGAGTAG

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT116568 WP_001190712.1 NZ_CP034789:c146874-146653 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT116568 NZ_CP034789:c146874-146653 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLP7

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References