Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 137457..139080 Replicon plasmid pTBMCR421
Accession NZ_CP034788
Organism Escherichia coli strain ECCNB20-2

Toxin (Protein)


Gene name hipA Uniprot ID H9TH67
Locus tag CRG85_RS24065 Protein ID WP_000088046.1
Coordinates 137457..138782 (-) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A5Z6V2D1
Locus tag CRG85_RS24070 Protein ID WP_024136327.1
Coordinates 138787..139080 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CRG85_RS24035 133441..134298 - 858 WP_000784387.1 HNH endonuclease -
CRG85_RS24040 134314..134622 - 309 WP_001224687.1 hypothetical protein -
CRG85_RS24045 135171..135596 - 426 WP_001043843.1 hypothetical protein -
CRG85_RS24050 135850..136665 - 816 WP_053276223.1 HNH endonuclease -
CRG85_RS24055 136678..137088 - 411 WP_000985911.1 hypothetical protein -
CRG85_RS24060 137190..137396 - 207 WP_000134171.1 hypothetical protein -
CRG85_RS24065 137457..138782 - 1326 WP_000088046.1 type II toxin-antitoxin system HipA family toxin Toxin
CRG85_RS24070 138787..139080 - 294 WP_024136327.1 helix-turn-helix transcriptional regulator Antitoxin
CRG85_RS24080 139684..140076 - 393 WP_000341066.1 hypothetical protein -
CRG85_RS24085 140449..140790 + 342 WP_001151575.1 hypothetical protein -
CRG85_RS24090 140882..141073 + 192 WP_000398480.1 hypothetical protein -
CRG85_RS24095 141123..141404 + 282 WP_000077926.1 hypothetical protein -
CRG85_RS24100 141967..143208 - 1242 WP_001053338.1 VWA domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aph(3')-Ia / sul3 / ant(3'')-Ia / cmlA1 / aadA2 / tet(M) / fosA3 / blaCTX-M-14 / aac(3)-IVa / aph(4)-Ia / sul2 / floR / mph(A) / mcr-1.1 - 1..254365 254365


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(9-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50249.20 Da        Isoelectric Point: 6.1927

>T116565 WP_000088046.1 NZ_CP034788:c138782-137457 [Escherichia coli]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

>T116565 NZ_CP034788:c138782-137457 [Escherichia coli]
ATGAGCCGGAAACAGCAACGTCTGGTAATTTGGATGAACGGCATCAAAGTCGGGTACTGGGAAAAAAGCAAAGGGGTAGA
CAGCTTAGAATACCTTCCTGAATGGGTTGCTGACGAACAGGGAAGGCCGCTGTCATTGTCCCTTCCTTTTACTCCCGGGA
ACCAGGTCTGGCGTGGAAATGTGGTACGTGACTATTTTGATAATTTACTGCCGGACAGCGAAGGCATACGCAGACGTCTG
GCAATGCGTTACAAGGCCGATAGCCTGGAGCCTTTTGATCTGCTGACGGAGCTGGGAAAAGACTGCGTGGGCGCGATACA
GCTGCTTCATGACGGAGATGAACCCACTGATTTATATTCCGTGAAGTATCACCCGCTTACTGAATCAGAAATTGCGGCAA
CTTTGCGTAATACCACGGAGACATTGCTACCGGGCAGGCCAGAAGATAACGACGACTTACGTTTATCTATTGCCGGTGCT
CAGGAGAAAACAGCCCTACTGTGGCATGAAGATCGGTGGTGTATGCCGGAGGGTAATACACCAACGACGCATATATTCAA
GCTACCGCTCGGACTTGTGGGGAACATGAAAGCCGACATGAGCTCGTCGGTTGAAAATGAATGGCTCTGCTCGGTACTTC
TCGGCCAGTACGGGCTCCCCGTGGCAAGAACGCAGATTGCTCACTTTGAAGATCAGAAAGCGCTGGTGGTAGAACGTTTT
GACCGAAAATGGTCAGGAGACGGACAATGGATCATTCGTTTACCCCAGGAGGATATGTGTCAGGCCCTGGGCGTTTCGCC
ATTACGAAAATATCAGGCGGACGGCGGGCCAGGTATATCGGAAATTATGGAGGTTCTGAGTAATTCAGACCGTGCTGAGC
GTGACAAAGCGCAGTTTTTTATGACGCAGATAATTTTCTGGATGATGGCCGCAACAGATGGCCACGCTAAAAATTTCAGT
ATTTCTATTGGGCCACAGGGACGTTATCACCTTACACCAAACTATGATGTGTTGTCGGCATGGCCGGTGATCGGTCATGG
GAACAATCAGATATCCTGGCAGAAGTGCAAACTGGCGATGGCCGTACGTGGCAGTAGCAACTATTACCAGATATATCGAA
TACAACGACGCCACTGGATCAGGCATGGTGAGATAACCGGCCTGAGTAAACAGCAGACAGAGGCGATGATAGAGGAAATC
ATCGCCAGAACCCCGGGTGTCATTGAACGTGTTAGCGGGCTTCTTCCGGACCAGTTCCCCCAGCAGCTTGCGGAAAGTAT
TTTTGATGGAATGAGGCAGCAATGCAGGCGCCTGGCTGAGAAATGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10946.35 Da        Isoelectric Point: 10.0925

>AT116565 WP_024136327.1 NZ_CP034788:c139080-138787 [Escherichia coli]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW

Download         Length: 294 bp

>AT116565 NZ_CP034788:c139080-138787 [Escherichia coli]
ATCAATAATGATTACCCGTTAAACACGCTCAACCAGCTGCGTCCCTTGCTCATTGGTTTTCGCAAGGCGAATGGCCTGAC
GCAAAAAGACCTGTCCGAAAGGTTAGGCGTCACTCAACAGACATACTCACGTCTGGAAGCCAACCCTGCCAGTGCGAGCA
TTGAACGGCTATTCAAGGTGTTTTCCATCCTGGGCGTAAAAATCAGCTTCTCCTCGGCAACCACCTCTTCAGAGAGGAAG
CAGACGGAAGAAATGCTTAAATCAAACTCACCTGCACGACAGGAGGATTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3T0VBU1

Antitoxin

Source ID Structure
AlphaFold DB A0A5Z6V2D1

References