Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4279002..4279499 Replicon chromosome
Accession NZ_CP034787
Organism Escherichia coli strain ECCNB20-2

Toxin (Protein)


Gene name ghoT Uniprot ID -
Locus tag CRG85_RS20735 Protein ID WP_077492245.1
Coordinates 4279002..4279175 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag CRG85_RS20740 Protein ID WP_000398619.1
Coordinates 4279203..4279499 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CRG85_RS20725 4275672..4277129 + 1458 WP_000856841.1 dipeptide/tripeptide permease DtpC -
CRG85_RS20730 4277366..4278883 + 1518 WP_001295074.1 lysine--tRNA ligase -
CRG85_RS20735 4279002..4279175 - 174 WP_077492245.1 type V toxin-antitoxin system toxin GhoT Toxin
CRG85_RS20740 4279203..4279499 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
CRG85_RS20745 4279726..4279998 - 273 WP_000405651.1 N-acetyltransferase -
CRG85_RS20750 4280010..4280240 - 231 WP_000371704.1 (4Fe-4S)-binding protein -
CRG85_RS20755 4280421..4282052 + 1632 WP_001216477.1 sensor histidine kinase -
CRG85_RS20760 4282049..4282768 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6631.16 Da        Isoelectric Point: 9.9031

>T116564 WP_077492245.1 NZ_CP034787:c4279175-4279002 [Escherichia coli]
MALFYKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T116564 NZ_CP034787:c4279175-4279002 [Escherichia coli]
ATGGCACTATTCTATAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT116564 WP_000398619.1 NZ_CP034787:c4279499-4279203 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT116564 NZ_CP034787:c4279499-4279203 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGACTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References