Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 3005144..3005979 Replicon chromosome
Accession NZ_CP034787
Organism Escherichia coli strain ECCNB20-2

Toxin (Protein)


Gene name yeeV Uniprot ID A0A7W4PV54
Locus tag CRG85_RS14660 Protein ID WP_000854821.1
Coordinates 3005144..3005521 (-) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A6H2GGB7
Locus tag CRG85_RS14665 Protein ID WP_001285390.1
Coordinates 3005611..3005979 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CRG85_RS14625 3000338..3002059 + 1722 WP_001202201.1 cysteine/glutathione ABC transporter ATP-binding protein/permease CydC -
CRG85_RS14630 3002101..3002805 + 705 WP_001241677.1 leucyl/phenylalanyl-tRNA--protein transferase -
CRG85_RS14635 3003090..3003308 + 219 WP_001040187.1 translation initiation factor IF-1 -
CRG85_RS14645 3003798..3004640 - 843 WP_072643849.1 DUF4942 domain-containing protein -
CRG85_RS14650 3004725..3004922 - 198 WP_023563519.1 DUF957 domain-containing protein -
CRG85_RS14655 3004950..3005147 - 198 Protein_2818 hypothetical protein -
CRG85_RS14660 3005144..3005521 - 378 WP_000854821.1 type IV toxin-antitoxin system toxin CbtA Toxin
CRG85_RS14665 3005611..3005979 - 369 WP_001285390.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
CRG85_RS14670 3006029..3006679 - 651 WP_113387941.1 antitoxin of toxin-antitoxin stability system -
CRG85_RS14675 3006692..3006913 - 222 WP_001544501.1 DUF987 domain-containing protein -
CRG85_RS14680 3006982..3007458 - 477 WP_001186742.1 RadC family protein -
CRG85_RS14685 3007473..3007958 - 486 WP_113387942.1 antirestriction protein -
CRG85_RS14690 3008049..3008867 - 819 WP_001234702.1 DUF945 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 13930.95 Da        Isoelectric Point: 8.5163

>T116558 WP_000854821.1 NZ_CP034787:c3005521-3005144 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPGAKQ

Download         Length: 378 bp

>T116558 NZ_CP034787:c3005521-3005144 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCACGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCATTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCACGCCGGGCAACCGGCCTGATGACACGCGA
CAACTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13811.52 Da        Isoelectric Point: 5.9618

>AT116558 WP_001285390.1 NZ_CP034787:c3005979-3005611 [Escherichia coli]
VSDTFHETNYPDDNNDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADSYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYMAVYPTPETKK

Download         Length: 369 bp

>AT116558 NZ_CP034787:c3005979-3005611 [Escherichia coli]
GTGTCAGACACGTTCCATGAAACAAATTATCCCGACGACAACAACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTTGGTGCCCGTCTGGTACAGGAAGGCAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGGCGGT
TCAGCGACGCGGATTCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAACATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGATACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7W4PV54

Antitoxin

Source ID Structure
AlphaFold DB A0A6H2GGB7

References