Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2454313..2454951 Replicon chromosome
Accession NZ_CP034787
Organism Escherichia coli strain ECCNB20-2

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag CRG85_RS12015 Protein ID WP_000813794.1
Coordinates 2454775..2454951 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag CRG85_RS12010 Protein ID WP_001270286.1
Coordinates 2454313..2454729 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CRG85_RS11990 2449465..2450406 - 942 WP_001251315.1 ABC transporter permease -
CRG85_RS11995 2450407..2451420 - 1014 WP_077491391.1 ABC transporter ATP-binding protein -
CRG85_RS12000 2451438..2452583 - 1146 WP_077491393.1 ABC transporter substrate-binding protein -
CRG85_RS12005 2452828..2454234 - 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
CRG85_RS12010 2454313..2454729 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
CRG85_RS12015 2454775..2454951 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
CRG85_RS12020 2455173..2455403 + 231 WP_000494244.1 YncJ family protein -
CRG85_RS12025 2455495..2457456 - 1962 WP_077491395.1 23S rRNA 5-hydroxycytidine C2501 synthase -
CRG85_RS12030 2457529..2458065 - 537 WP_000429150.1 DNA-binding transcriptional regulator SutR -
CRG85_RS12035 2458118..2459332 + 1215 WP_072169390.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T116557 WP_000813794.1 NZ_CP034787:c2454951-2454775 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T116557 NZ_CP034787:c2454951-2454775 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT116557 WP_001270286.1 NZ_CP034787:c2454729-2454313 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT116557 NZ_CP034787:c2454729-2454313 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References