Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 1005543..1006126 Replicon chromosome
Accession NZ_CP034787
Organism Escherichia coli strain ECCNB20-2

Toxin (Protein)


Gene name mazF Uniprot ID S1EZP4
Locus tag CRG85_RS04880 Protein ID WP_000254738.1
Coordinates 1005791..1006126 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A1V2T5M9
Locus tag CRG85_RS04875 Protein ID WP_000581941.1
Coordinates 1005543..1005791 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CRG85_RS04865 1001882..1003183 + 1302 WP_077492052.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
CRG85_RS04870 1003231..1005465 + 2235 WP_000226815.1 GTP pyrophosphokinase -
CRG85_RS04875 1005543..1005791 + 249 WP_000581941.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
CRG85_RS04880 1005791..1006126 + 336 WP_000254738.1 endoribonuclease MazF Toxin
CRG85_RS04885 1006197..1006988 + 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
CRG85_RS04890 1007216..1008853 + 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
CRG85_RS04895 1008941..1010239 + 1299 WP_000036723.1 phosphopyruvate hydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12098.04 Da        Isoelectric Point: 8.2618

>T116545 WP_000254738.1 NZ_CP034787:1005791-1006126 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T116545 NZ_CP034787:1005791-1006126 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9385.76 Da        Isoelectric Point: 4.4665

>AT116545 WP_000581941.1 NZ_CP034787:1005543-1005791 [Escherichia coli]
MIHSSVKRWGNSPAVRIPTTLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT116545 NZ_CP034787:1005543-1005791 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGACTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CK9
PDB 5CKH
PDB 5CO7
PDB 3NFC
PDB 5CR2
PDB 5CKB
PDB 5CQY
PDB 5CKD
PDB 5CKE
PDB 5CKF

Antitoxin

Source ID Structure
AlphaFold DB A0A1V2T5M9

References