Detailed information of TA system TA116542

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 85447..85973
• Replicon: plasmid pTB402
• Accession: NZ_CP034786
• Organism: Escherichia coli strain ECZP248

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: ENE68_RS23720
• Protein ID: WP_000323025.1
• Coordinates: 85686..85973 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: ENE68_RS23715
• Protein ID: WP_000534858.1
• Coordinates: 85447..85686 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ENE68_RS23690 (ENE68_23690)	81506..82203	+	698	WP_103215986.1	IS1-like element IS1A family transposase	-
ENE68_RS23695 (ENE68_23695)	82530..82902	+	373	Protein_87	IS1 family transposase	-
ENE68_RS23705 (ENE68_23705)	83058..83762	-	705	WP_001067852.1	IS6-like element IS26 family transposase	-
ENE68_RS23710 (ENE68_23710)	83839..84912	-	1074	WP_000557466.1	hypothetical protein	-
ENE68_RS24275	84956..85105	-	150	WP_000550473.1	hypothetical protein	-
ENE68_RS24735	85312..85422	-	111	Protein_91	protein YdfV	-
ENE68_RS23715 (ENE68_23715)	85447..85686	+	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
ENE68_RS23720 (ENE68_23720)	85686..85973	+	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
ENE68_RS23730 (ENE68_23730)	86256..86480	+	225	Protein_94	hypothetical protein	-
ENE68_RS23735 (ENE68_23735)	86917..87126	+	210	WP_072662162.1	hypothetical protein	-
ENE68_RS23740 (ENE68_23740)	87132..87621	-	490	Protein_96	IS3 family transposase	-
ENE68_RS23745 (ENE68_23745)	87702..89210	-	1509	WP_127762875.1	group II intron reverse transcriptase/maturase	-
ENE68_RS23755 (ENE68_23755)	89883..90308	-	426	Protein_98	IS3 family transposase	-
ENE68_RS23760 (ENE68_23760)	90296..90376	+	81	Protein_99	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / aadA5 / floR	-	1..143706	143706
	-	inside	IScluster/Tn	-	-	83058..91803	8745

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T116542 WP_000323025.1 NZ_CP034786:85686-85973 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T116542 NZ_CP034786:85686-85973 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT116542 WP_000534858.1 NZ_CP034786:85447-85686 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT116542 NZ_CP034786:85447-85686 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGCGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• PDB: 4FXE
• PDB: 2KC8
• PDB: 2K29
• AlphaFold DB: A0A4V1CTS8