Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 8199..8800 Replicon plasmid pTB402
Accession NZ_CP034786
Organism Escherichia coli strain ECZP248

Toxin (Protein)


Gene name doc Uniprot ID A0A838AVV6
Locus tag ENE68_RS23265 Protein ID WP_063501799.1
Coordinates 8420..8800 (+) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag ENE68_RS23260 Protein ID WP_001190712.1
Coordinates 8199..8420 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ENE68_RS23250 (ENE68_23250) 5226..6463 - 1238 Protein_4 restriction endonuclease subunit S -
ENE68_RS23255 (ENE68_23255) 6460..8016 - 1557 WP_063501802.1 type I restriction-modification system subunit M -
ENE68_RS23260 (ENE68_23260) 8199..8420 + 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
ENE68_RS23265 (ENE68_23265) 8420..8800 + 381 WP_063501799.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
ENE68_RS24695 (ENE68_23270) 8805..8984 + 180 WP_001411052.1 hypothetical protein -
ENE68_RS23275 (ENE68_23275) 9012..10055 + 1044 WP_063501800.1 DUF968 domain-containing protein -
ENE68_RS23280 (ENE68_23280) 10198..10895 + 698 WP_094096600.1 IS1-like element IS1A family transposase -
ENE68_RS23285 (ENE68_23285) 11085..11306 + 222 WP_001361625.1 host cell division inhibitor Icd-like protein -
ENE68_RS23290 (ENE68_23290) 11303..12421 + 1119 Protein_12 phage antirepressor KilAC domain-containing protein -
ENE68_RS23295 (ENE68_23295) 12454..13305 - 852 WP_000611656.1 phage repressor protein C1 -
ENE68_RS23300 (ENE68_23300) 13416..13625 - 210 WP_063501801.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / aadA5 / floR - 1..143706 143706


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(4-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13616.39 Da        Isoelectric Point: 5.6343

>T116540 WP_063501799.1 NZ_CP034786:8420-8800 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVVYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGTATGEISVSTVAATLRRLYGSAE

Download         Length: 381 bp

>T116540 NZ_CP034786:8420-8800 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATAGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCTGGCATGTC
AGATCCGGGCAGGGCAGAGGCCATTATCGGGAGAGTCCAGGCCAGAGTTGTGTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGCTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGGACTGCGACTGG
CGAGATATCTGTATCTACTGTCGCCGCCACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT116540 WP_001190712.1 NZ_CP034786:8199-8420 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT116540 NZ_CP034786:8199-8420 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A838AVV6

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References