Detailed information of TA system TA116537

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3862896..3863728
• Replicon: chromosome
• Accession: NZ_CP034784
• Organism: Escherichia coli strain ECZP248

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A0Q2Y5N3
• Locus tag: ENE68_RS18965
• Protein ID: WP_001514886.1
• Coordinates: 3862896..3863270 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: ENE68_RS18970
• Protein ID: WP_127762847.1
• Coordinates: 3863360..3863728 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ENE68_RS24180 (3860624)	3860624..3860773	-	150	Protein_3702	hypothetical protein	-
ENE68_RS18945 (3860879)	3860879..3861055	-	177	WP_000839290.1	DUF957 domain-containing protein	-
ENE68_RS18950 (3861072)	3861072..3861434	-	363	Protein_3704	DUF5983 family protein	-
ENE68_RS18955 (3861528)	3861528..3862756	+	1229	WP_155105063.1	IS3 family transposase	-
ENE68_RS24570 (3862768)	3862768..3862899	-	132	Protein_3706	DUF5983 family protein	-
ENE68_RS18965 (3862896)	3862896..3863270	-	375	WP_001514886.1	TA system toxin CbtA family protein	Toxin
ENE68_RS18970 (3863360)	3863360..3863728	-	369	WP_127762847.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
ENE68_RS18975 (3863891)	3863891..3864112	-	222	WP_000692286.1	DUF987 domain-containing protein	-
ENE68_RS18980 (3864175)	3864175..3864651	-	477	WP_001186774.1	RadC family protein	-
ENE68_RS18985 (3864667)	3864667..3865149	-	483	WP_001508976.1	antirestriction protein	-
ENE68_RS18990 (3865241)	3865241..3866059	-	819	WP_001508975.1	DUF932 domain-containing protein	-
ENE68_RS19000 (3866177)	3866177..3866372	-	196	Protein_3713	DUF905 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13930.91 Da
• Isoelectric Point: 7.2909

>T116537 WP_001514886.1 NZ_CP034784:c3863270-3862896 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYGLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQSGF
SAGASSQLINSIDILRARRATGLMTRHNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T116537 NZ_CP034784:c3863270-3862896 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGTGGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGTCGGGATTC
AGCGCAGGAGCCTCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCCGCCA
CAACTACAGAACGGTGAACAATATCACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13609.38 Da
• Isoelectric Point: 5.9598

>AT116537 WP_127762847.1 NZ_CP034784:c3863728-3863360 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLLCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT116537 NZ_CP034784:c3863728-3863360 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACTCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A0Q2Y5N3