Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 3822564..3823140 Replicon chromosome
Accession NZ_CP034784
Organism Escherichia coli strain ECZP248

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag ENE68_RS18760 Protein ID WP_001297643.1
Coordinates 3822853..3823140 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A080I7H7
Locus tag ENE68_RS18755 Protein ID WP_000063160.1
Coordinates 3822564..3822866 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ENE68_RS18740 (3819160) 3819160..3821310 + 2151 WP_001340937.1 pyruvate/proton symporter BtsT -
ENE68_RS18745 (3821360) 3821360..3821563 + 204 WP_000467859.1 YbdD/YjiX family protein -
ENE68_RS18750 (3821574) 3821574..3822530 + 957 WP_001297640.1 GTPase -
ENE68_RS18755 (3822564) 3822564..3822866 - 303 WP_000063160.1 BrnA antitoxin family protein Antitoxin
ENE68_RS18760 (3822853) 3822853..3823140 - 288 WP_001297643.1 BrnT family toxin Toxin
ENE68_RS24880 (3823339) 3823339..3824035 - 697 Protein_3667 IS1 family transposase -
ENE68_RS18770 (3824350) 3824350..3825582 + 1233 WP_001137031.1 multidrug efflux MFS transporter MdtM -
ENE68_RS18775 (3825623) 3825623..3826903 + 1281 WP_001037390.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 3823339..3823548 209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T116536 WP_001297643.1 NZ_CP034784:c3823140-3822853 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T116536 NZ_CP034784:c3823140-3822853 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11552.01 Da        Isoelectric Point: 9.4646

>AT116536 WP_000063160.1 NZ_CP034784:c3822866-3822564 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALTNKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT116536 NZ_CP034784:c3822866-3822564 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGACGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A080I7H7

References