Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3303247..3303865 Replicon chromosome
Accession NZ_CP034784
Organism Escherichia coli strain ECZP248

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag ENE68_RS16140 Protein ID WP_001291435.1
Coordinates 3303647..3303865 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag ENE68_RS16135 Protein ID WP_000344800.1
Coordinates 3303247..3303621 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ENE68_RS16125 (3298336) 3298336..3299529 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
ENE68_RS16130 (3299552) 3299552..3302701 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
ENE68_RS16135 (3303247) 3303247..3303621 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
ENE68_RS16140 (3303647) 3303647..3303865 + 219 WP_001291435.1 HHA domain-containing protein Toxin
ENE68_RS16145 (3304037) 3304037..3304588 + 552 WP_000102564.1 maltose O-acetyltransferase -
ENE68_RS16150 (3304704) 3304704..3305174 + 471 WP_000136192.1 YlaC family protein -
ENE68_RS16155 (3305338) 3305338..3306888 + 1551 WP_023281027.1 cyclic-guanylate-specific phosphodiesterase PdeB -
ENE68_RS16160 (3306930) 3306930..3307217 - 288 Protein_3157 DUF1428 family protein -
ENE68_RS16165 (3307265) 3307265..3307962 + 698 WP_095033700.1 IS1-like element IS1B family transposase -
ENE68_RS16170 (3308177) 3308177..3308644 - 468 WP_001074470.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 3307585..3307962 377


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T116533 WP_001291435.1 NZ_CP034784:3303647-3303865 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T116533 NZ_CP034784:3303647-3303865 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT116533 WP_000344800.1 NZ_CP034784:3303247-3303621 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT116533 NZ_CP034784:3303247-3303621 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References