Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2324721..2325359 Replicon chromosome
Accession NZ_CP034784
Organism Escherichia coli strain ECZP248

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag ENE68_RS11300 Protein ID WP_000813794.1
Coordinates 2325183..2325359 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag ENE68_RS11295 Protein ID WP_001270286.1
Coordinates 2324721..2325137 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ENE68_RS11275 (2319873) 2319873..2320814 - 942 WP_001251319.1 ABC transporter permease -
ENE68_RS11280 (2320815) 2320815..2321828 - 1014 WP_000220393.1 ABC transporter ATP-binding protein -
ENE68_RS11285 (2321846) 2321846..2322991 - 1146 WP_000047418.1 ABC transporter substrate-binding protein -
ENE68_RS11290 (2323236) 2323236..2324642 - 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
ENE68_RS11295 (2324721) 2324721..2325137 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
ENE68_RS11300 (2325183) 2325183..2325359 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
ENE68_RS11305 (2325581) 2325581..2325811 + 231 WP_000494244.1 YncJ family protein -
ENE68_RS11310 (2325903) 2325903..2327864 - 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
ENE68_RS11315 (2327937) 2327937..2328473 - 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
ENE68_RS11320 (2328565) 2328565..2329740 + 1176 WP_032253412.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T116532 WP_000813794.1 NZ_CP034784:c2325359-2325183 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T116532 NZ_CP034784:c2325359-2325183 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT116532 WP_001270286.1 NZ_CP034784:c2325137-2324721 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT116532 NZ_CP034784:c2325137-2324721 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References