Detailed information of TA system TA116509

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 26478..27070
• Replicon: plasmid pMPC6-47K
• Accession: NZ_CP034781
• Organism: Pseudomonas sp. MPC6

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: A0A5B7VRE9
• Locus tag: ELQ88_RS00170
• Protein ID: WP_016715863.1
• Coordinates: 26768..27070 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: ELQ88_RS00165
• Protein ID: WP_016715864.1
• Coordinates: 26478..26771 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ELQ88_RS00145	22474..24096	+	1623	WP_138962786.1	type I restriction-modification system subunit M	-
ELQ88_RS00150	24728..25561	+	834	WP_106117669.1	DUF945 domain-containing protein	-
ELQ88_RS00155	25616..26074	+	459	WP_138962788.1	hypothetical protein	-
ELQ88_RS00160	26127..26429	+	303	WP_138962790.1	hypothetical protein	-
ELQ88_RS00165	26478..26771	-	294	WP_016715864.1	putative addiction module antidote protein	Antitoxin
ELQ88_RS00170	26768..27070	-	303	WP_016715863.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ELQ88_RS00175	27248..27427	+	180	WP_138962792.1	hypothetical protein	-
ELQ88_RS00180	27487..27939	+	453	WP_138962794.1	hypothetical protein	-
ELQ88_RS00190	28796..29668	-	873	WP_027618234.1	oxaloacetate decarboxylase	-
ELQ88_RS00195	29923..30531	-	609	WP_027618235.1	L(+)-tartrate dehydratase subunit beta	-
ELQ88_RS00200	30528..31427	-	900	WP_027618236.1	L(+)-tartrate dehydratase subunit alpha	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..47564	47564

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Uncharacterized protein HI1420 (8-94)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11268.89 Da
• Isoelectric Point: 9.5014

>T116509 WP_016715863.1 NZ_CP034781:c27070-26768 [Pseudomonas sp. MPC6]
MKTIKQTATYMTWERKLRDQRAKAIIAARVFRVANGLLGDVEPVGQGVSELRIHYGPGYRVYFQQRGNELVLLLCGGDKG
SQARDIETAKLLASQWSEDE

Nucleotide

• Length: 303 bp

>T116509 NZ_CP034781:c27070-26768 [Pseudomonas sp. MPC6]
ATGAAAACGATCAAACAAACCGCTACTTACATGACGTGGGAACGCAAGCTGAGAGATCAGCGGGCAAAAGCGATCATCGC
GGCGCGGGTCTTCCGGGTAGCCAACGGCCTATTGGGCGATGTTGAGCCGGTCGGCCAGGGTGTTAGCGAGTTGCGTATTC
ACTACGGCCCCGGCTACCGGGTTTATTTTCAGCAGCGTGGTAACGAGCTTGTATTGCTGCTGTGTGGTGGCGACAAAGGC
AGCCAGGCCCGCGATATCGAAACCGCCAAACTGCTTGCCAGCCAGTGGAGTGAGGATGAATGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10519.09 Da
• Isoelectric Point: 6.2189

>AT116509 WP_016715864.1 NZ_CP034781:c26771-26478 [Pseudomonas sp. MPC6]
MKEPIYDYDPAAALVDPETIALFMADAFETGDAGHIAKALGVVARAKGMTELAKESGLSREQLYRSFSDKGNPTLKTMLA
VMRAVGLDLTTRQHVHP

Nucleotide

• Length: 294 bp

>AT116509 NZ_CP034781:c26771-26478 [Pseudomonas sp. MPC6]
ATGAAAGAGCCAATCTATGACTATGATCCAGCGGCTGCGCTGGTCGATCCTGAAACCATCGCCCTGTTCATGGCCGATGC
TTTTGAAACCGGTGACGCTGGCCATATCGCTAAAGCCCTCGGCGTAGTCGCTCGCGCCAAAGGCATGACAGAGCTGGCCA
AAGAGTCGGGGCTATCCCGCGAACAGCTTTACCGCTCGTTCAGCGACAAGGGCAACCCTACGTTGAAAACCATGCTCGCA
GTGATGCGCGCCGTTGGCCTGGATCTGACCACACGGCAACACGTCCACCCCTGA

Structures

Toxin

• AlphaFold DB: A0A5B7VRE9