116508

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5210492..5211117 Replicon chromosome
Accession NZ_CP034778
Organism Klebsiella pneumoniae strain 18CPO060

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag ENZ43_RS27580 Protein ID WP_002882817.1
Coordinates 5210492..5210875 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag ENZ43_RS27585 Protein ID WP_004150355.1
Coordinates 5210875..5211117 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ENZ43_RS27565 5207858..5208760 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
ENZ43_RS27570 5208757..5209392 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
ENZ43_RS27575 5209389..5210318 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
ENZ43_RS27580 5210492..5210875 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
ENZ43_RS27585 5210875..5211117 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
ENZ43_RS27590 5211322..5212239 + 918 WP_004213381.1 alpha/beta hydrolase -
ENZ43_RS27595 5212254..5213195 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
ENZ43_RS27600 5213240..5213677 - 438 WP_023302745.1 D-tyrosyl-tRNA(Tyr) deacylase -
ENZ43_RS27605 5213674..5214534 - 861 WP_004146232.1 virulence factor BrkB family protein -
ENZ43_RS27610 5214528..5215127 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T116508 WP_002882817.1 NZ_CP034778:c5210875-5210492 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T116508 NZ_CP034778:c5210875-5210492 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACTTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT116508 WP_004150355.1 NZ_CP034778:c5211117-5210875 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT116508 NZ_CP034778:c5211117-5210875 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References