Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 4733008..4733524 Replicon chromosome
Accession NZ_CP034778
Organism Klebsiella pneumoniae strain 18CPO060

Toxin (Protein)


Gene name relE Uniprot ID R4YAY3
Locus tag ENZ43_RS25230 Protein ID WP_004178374.1
Coordinates 4733008..4733292 (-) Length 95 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID R4Y888
Locus tag ENZ43_RS25235 Protein ID WP_002886901.1
Coordinates 4733282..4733524 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ENZ43_RS25210 4728491..4728799 - 309 WP_004210629.1 PTS sugar transporter subunit IIB -
ENZ43_RS28635 4728884..4729057 + 174 WP_004210630.1 hypothetical protein -
ENZ43_RS25215 4729060..4729803 + 744 WP_002886905.1 MurR/RpiR family transcriptional regulator -
ENZ43_RS25220 4730160..4732298 + 2139 WP_004186701.1 anaerobic ribonucleoside-triphosphate reductase -
ENZ43_RS25225 4732540..4733004 + 465 WP_004178375.1 anaerobic ribonucleoside-triphosphate reductase-activating protein -
ENZ43_RS25230 4733008..4733292 - 285 WP_004178374.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
ENZ43_RS25235 4733282..4733524 - 243 WP_002886901.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
ENZ43_RS25240 4733602..4735512 - 1911 WP_004152270.1 BglG family transcription antiterminator -
ENZ43_RS25245 4735535..4736689 - 1155 WP_004210635.1 lactonase family protein -
ENZ43_RS25250 4736756..4737496 - 741 WP_004186692.1 KDGP aldolase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-83)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-80)


Sequences

Toxin        


Download         Length: 95 a.a.        Molecular weight: 11155.97 Da        Isoelectric Point: 10.3787

>T116506 WP_004178374.1 NZ_CP034778:c4733292-4733008 [Klebsiella pneumoniae]
MTYELEFDPRAWREWQKPGETVKKQFKNKLQQIVQNPRIESTRLSDLPDCYKIKLKASGYRLVYQVRDSVVVVYVIAIGK
REKAAVYHQANKRL

Download         Length: 285 bp

>T116506 NZ_CP034778:c4733292-4733008 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9045.55 Da        Isoelectric Point: 6.3340

>AT116506 WP_002886901.1 NZ_CP034778:c4733524-4733282 [Klebsiella pneumoniae]
MATLNVRLDDKLKKEAYAVLEKLDMTPTEAVRLLFQYIAENGRMPVKSMTISDGEAALLRTVRERLENPRPGVKVSLDDL

Download         Length: 243 bp

>AT116506 NZ_CP034778:c4733524-4733282 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCAGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A6THG1

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GLP0

References