Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 21103..21628 Replicon plasmid pKPCKP060
Accession NZ_CP034777
Organism Klebsiella pneumoniae strain 18CPO060

Toxin (Protein)


Gene name ccdB Uniprot ID W8UQV6
Locus tag ENZ43_RS01345 Protein ID WP_001568026.1
Coordinates 21323..21628 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID W8V2V6
Locus tag ENZ43_RS01340 Protein ID WP_001568025.1
Coordinates 21103..21321 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ENZ43_RS01290 16286..16516 + 231 Protein_14 IS5/IS1182 family transposase -
ENZ43_RS01295 16688..17047 + 360 WP_004152400.1 DUF305 domain-containing protein -
ENZ43_RS01300 17704..18114 - 411 WP_004152401.1 hypothetical protein -
ENZ43_RS01305 18213..18353 - 141 Protein_17 LacI family DNA-binding transcriptional regulator -
ENZ43_RS01310 18357..19054 - 698 Protein_18 IS1 family transposase -
ENZ43_RS01315 19088..19798 - 711 WP_004152334.1 ATP-binding protein -
ENZ43_RS01320 19872..20288 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin -
ENZ43_RS01325 20285..20515 - 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
ENZ43_RS28160 20472..20933 + 462 WP_162589881.1 hypothetical protein -
ENZ43_RS01340 21103..21321 + 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
ENZ43_RS01345 21323..21628 + 306 WP_001568026.1 type II toxin-antitoxin system toxin CcdB Toxin
ENZ43_RS01350 21797..22192 + 396 WP_072158608.1 hypothetical protein -
ENZ43_RS01355 22219..22542 + 324 WP_022644730.1 hypothetical protein -
ENZ43_RS01360 22539..23555 + 1017 WP_020315256.1 hypothetical protein -
ENZ43_RS01365 23890..25158 - 1269 WP_019705993.1 ISL3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaKPC-2 - 1..109503 109503
- inside IScluster/Tn blaKPC-2 - 7048..25209 18161


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11645.32 Da        Isoelectric Point: 5.6919

>T116490 WP_001568026.1 NZ_CP034777:21323-21628 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASAWLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T116490 NZ_CP034777:21323-21628 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCTGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGACGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8392.34 Da        Isoelectric Point: 4.7405

>AT116490 WP_001568025.1 NZ_CP034777:21103-21321 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT116490 NZ_CP034777:21103-21321 [Klebsiella pneumoniae]
ATGAAGCAGCGCATCACTGTCACCATAGACAGTGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A514EZN1

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4ZZP8

References