Detailed information of TA system TA116489

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 19872..20515
• Replicon: plasmid pKPCKP060
• Accession: NZ_CP034777
• Organism: Klebsiella pneumoniae strain 18CPO060

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: D8L2J9
• Locus tag: ENZ43_RS01320
• Protein ID: WP_001044770.1
• Coordinates: 19872..20288 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D5KTK7
• Locus tag: ENZ43_RS01325
• Protein ID: WP_001261282.1
• Coordinates: 20285..20515 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ENZ43_RS01285	15000..16184	-	1185	WP_004152398.1	ISAs1-like element ISKpn31 family transposase	-
ENZ43_RS01290	16286..16516	+	231	Protein_14	IS5/IS1182 family transposase	-
ENZ43_RS01295	16688..17047	+	360	WP_004152400.1	DUF305 domain-containing protein	-
ENZ43_RS01300	17704..18114	-	411	WP_004152401.1	hypothetical protein	-
ENZ43_RS01305	18213..18353	-	141	Protein_17	LacI family DNA-binding transcriptional regulator	-
ENZ43_RS01310	18357..19054	-	698	Protein_18	IS1 family transposase	-
ENZ43_RS01315	19088..19798	-	711	WP_004152334.1	ATP-binding protein	-
ENZ43_RS01320	19872..20288	-	417	WP_001044770.1	type II toxin-antitoxin system VapC family toxin	Toxin
ENZ43_RS01325	20285..20515	-	231	WP_001261282.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
ENZ43_RS28160	20472..20933	+	462	WP_162589881.1	hypothetical protein	-
ENZ43_RS01340	21103..21321	+	219	WP_001568025.1	type II toxin-antitoxin system antitoxin CcdA	-
ENZ43_RS01345	21323..21628	+	306	WP_001568026.1	type II toxin-antitoxin system toxin CcdB	-
ENZ43_RS01350	21797..22192	+	396	WP_072158608.1	hypothetical protein	-
ENZ43_RS01355	22219..22542	+	324	WP_022644730.1	hypothetical protein	-
ENZ43_RS01360	22539..23555	+	1017	WP_020315256.1	hypothetical protein	-
ENZ43_RS01365	23890..25158	-	1269	WP_019705993.1	ISL3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaKPC-2	-	1..109503	109503
	-	inside	IScluster/Tn	blaKPC-2	-	7048..25209	18161

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15106.52 Da
• Isoelectric Point: 7.1084

>T116489 WP_001044770.1 NZ_CP034777:c20288-19872 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T116489 NZ_CP034777:c20288-19872 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8756.83 Da
• Isoelectric Point: 4.4692

>AT116489 WP_001261282.1 NZ_CP034777:c20515-20285 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT116489 NZ_CP034777:c20515-20285 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: A0A0H3GYM2

Antitoxin

• AlphaFold DB: A0A0H3GZG3