Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 140666..141576 Replicon plasmid phvKP060
Accession NZ_CP034776
Organism Klebsiella pneumoniae strain 18CPO060

Toxin (Protein)


Gene name - Uniprot ID A0A7D3P3P5
Locus tag ENZ43_RS00805 Protein ID WP_023302799.1
Coordinates 140666..141136 (-) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A6P1V3Q9
Locus tag ENZ43_RS00810 Protein ID WP_004026357.1
Coordinates 141133..141576 (-) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ENZ43_RS00785 136193..136591 - 399 WP_004212796.1 H-NS histone family protein -
ENZ43_RS00790 136928..137422 - 495 WP_004212794.1 thermonuclease family protein -
ENZ43_RS00795 137600..138549 + 950 Protein_148 DUF1173 family protein -
ENZ43_RS00800 139935..140632 - 698 Protein_149 IS1 family transposase -
ENZ43_RS00805 140666..141136 - 471 WP_023302799.1 RES family NAD+ phosphorylase Toxin
ENZ43_RS00810 141133..141576 - 444 WP_004026357.1 DUF2384 domain-containing protein Antitoxin
ENZ43_RS00815 141677..142180 - 504 Protein_152 DUF4113 domain-containing protein -
ENZ43_RS00820 142278..142499 - 222 WP_004213643.1 DUF2188 domain-containing protein -
ENZ43_RS00825 142644..142799 - 156 WP_004213642.1 hypothetical protein -
ENZ43_RS00830 142874..145000 - 2127 WP_004213640.1 TonB-dependent receptor family protein -
ENZ43_RS00835 145087..146040 - 954 Protein_156 fec operon regulator FecR -
ENZ43_RS00840 146037..146558 - 522 WP_004213635.1 sigma-70 family RNA polymerase sigma factor -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - rmpA2 / rmpA / iutA / iucD / iucC / iucB / iucA / iroB / iroC / iroD / iroN / rmpA 1..218147 218147
- flank IS/Tn - - 139935..140438 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-138)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(30-89)


Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 17461.84 Da        Isoelectric Point: 4.4411

>T116488 WP_023302799.1 NZ_CP034776:c141136-140666 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIVWVHHILVMLPTY

Download         Length: 471 bp

>T116488 NZ_CP034776:c141136-140666 [Klebsiella pneumoniae]
GTGATCCTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
TTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACGCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTGATCCAAAGCGCAAACATGGAT
GAATTACCGGGCAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAACTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATTGTATGGGTCCACCACATATTGGTGATGCTGCCAACTTACTGA

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16480.80 Da        Isoelectric Point: 10.2498

>AT116488 WP_004026357.1 NZ_CP034776:c141576-141133 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDKEAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp

>AT116488 NZ_CP034776:c141576-141133 [Klebsiella pneumoniae]
ATGAAAACATTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
CGTAGCGCTTCTGGGCCAAATCAATGAAGGCCTGGACGGGAAGGTGGCGAATCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCGACCACGTTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGGATTATCCGCGTCATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAAGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAGACAGGAGCGTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7D3P3P5

Antitoxin

Source ID Structure
AlphaFold DB A0A6P1V3Q9

References