Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 103584..104320 Replicon plasmid phvKP060
Accession NZ_CP034776
Organism Klebsiella pneumoniae strain 18CPO060

Toxin (Protein)


Gene name tacT Uniprot ID A0A2J5Q928
Locus tag ENZ43_RS00610 Protein ID WP_004098919.1
Coordinates 103838..104320 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID A0A7D3T1D0
Locus tag ENZ43_RS00605 Protein ID WP_004213599.1
Coordinates 103584..103850 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ENZ43_RS00580 98816..99373 + 558 WP_004213590.1 recombinase family protein -
ENZ43_RS00585 99376..102345 + 2970 WP_004213592.1 Tn3 family transposase -
ENZ43_RS00590 102387..102881 + 495 WP_004213594.1 hypothetical protein -
ENZ43_RS00595 102942..103145 + 204 WP_004213596.1 hemolysin expression modulator Hha -
ENZ43_RS00600 103159..103389 + 231 WP_004213598.1 hypothetical protein -
ENZ43_RS00605 103584..103850 + 267 WP_004213599.1 DUF1778 domain-containing protein Antitoxin
ENZ43_RS00610 103838..104320 + 483 WP_004098919.1 GNAT family N-acetyltransferase Toxin
ENZ43_RS00615 104741..105968 + 1228 Protein_113 IS3 family transposase -
ENZ43_RS00620 105964..106359 - 396 Protein_114 DDE-type integrase/transposase/recombinase -
ENZ43_RS00625 106534..107556 - 1023 WP_004214536.1 porphobilinogen synthase -
ENZ43_RS00630 107620..108966 - 1347 WP_011154555.1 dihydroorotase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - rmpA2 / rmpA / iutA / iucD / iucC / iucB / iucA / iroB / iroC / iroD / iroN / rmpA 1..218147 218147
- inside IScluster/Tn - - 98816..105968 7152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(66-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17335.06 Da        Isoelectric Point: 10.0704

>T116487 WP_004098919.1 NZ_CP034776:103838-104320 [Klebsiella pneumoniae]
VGRITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKKGTKKVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T116487 NZ_CP034776:103838-104320 [Klebsiella pneumoniae]
GTGGGACGTATAACCGCGCCCGAGCCGTTATCCAGCGCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCACTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGAAGGGTACGA
AGAAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCTACAGGCAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGTGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGCGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10151.58 Da        Isoelectric Point: 4.5141

>AT116487 WP_004213599.1 NZ_CP034776:103584-103850 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRVLIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDDSAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT116487 NZ_CP034776:103584-103850 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGTACTCATCGATGCCGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGATTCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J5Q928

Antitoxin

Source ID Structure
AlphaFold DB A0A7D3T1D0

References