Detailed information of TA system TA116487

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 103584..104320
• Replicon: plasmid phvKP060
• Accession: NZ_CP034776
• Organism: Klebsiella pneumoniae strain 18CPO060

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A2J5Q928
• Locus tag: ENZ43_RS00610
• Protein ID: WP_004098919.1
• Coordinates: 103838..104320 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: A0A7D3T1D0
• Locus tag: ENZ43_RS00605
• Protein ID: WP_004213599.1
• Coordinates: 103584..103850 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ENZ43_RS00580	98816..99373	+	558	WP_004213590.1	recombinase family protein	-
ENZ43_RS00585	99376..102345	+	2970	WP_004213592.1	Tn3 family transposase	-
ENZ43_RS00590	102387..102881	+	495	WP_004213594.1	hypothetical protein	-
ENZ43_RS00595	102942..103145	+	204	WP_004213596.1	hemolysin expression modulator Hha	-
ENZ43_RS00600	103159..103389	+	231	WP_004213598.1	hypothetical protein	-
ENZ43_RS00605	103584..103850	+	267	WP_004213599.1	DUF1778 domain-containing protein	Antitoxin
ENZ43_RS00610	103838..104320	+	483	WP_004098919.1	GNAT family N-acetyltransferase	Toxin
ENZ43_RS00615	104741..105968	+	1228	Protein_113	IS3 family transposase	-
ENZ43_RS00620	105964..106359	-	396	Protein_114	DDE-type integrase/transposase/recombinase	-
ENZ43_RS00625	106534..107556	-	1023	WP_004214536.1	porphobilinogen synthase	-
ENZ43_RS00630	107620..108966	-	1347	WP_011154555.1	dihydroorotase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	rmpA2 / rmpA / iutA / iucD / iucC / iucB / iucA / iroB / iroC / iroD / iroN / rmpA	1..218147	218147
	-	inside	IScluster/Tn	-	-	98816..105968	7152

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (66-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17335.06 Da
• Isoelectric Point: 10.0704

>T116487 WP_004098919.1 NZ_CP034776:103838-104320 [Klebsiella pneumoniae]
VGRITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKKGTKKVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T116487 NZ_CP034776:103838-104320 [Klebsiella pneumoniae]
GTGGGACGTATAACCGCGCCCGAGCCGTTATCCAGCGCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCACTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGAAGGGTACGA
AGAAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCTACAGGCAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGTGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGCGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10151.58 Da
• Isoelectric Point: 4.5141

>AT116487 WP_004213599.1 NZ_CP034776:103584-103850 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRVLIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDDSAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT116487 NZ_CP034776:103584-103850 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGTACTCATCGATGCCGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGATTCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Structures

Toxin

• AlphaFold DB: A0A2J5Q928

Antitoxin

• AlphaFold DB: A0A7D3T1D0