Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 38236..38906 Replicon plasmid phvKP060
Accession NZ_CP034776
Organism Klebsiella pneumoniae strain 18CPO060

Toxin (Protein)


Gene name vagD Uniprot ID Q6U619
Locus tag ENZ43_RS00250 Protein ID WP_004213072.1
Coordinates 38463..38906 (+) Length 148 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q6U620
Locus tag ENZ43_RS00245 Protein ID WP_004213073.1
Coordinates 38236..38466 (+) Length 77 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ENZ43_RS00220 34289..35257 + 969 WP_011154535.1 IS5 family transposase -
ENZ43_RS00225 35348..35638 - 291 WP_004213078.1 hypothetical protein -
ENZ43_RS00230 35990..36196 + 207 WP_004213077.1 hypothetical protein -
ENZ43_RS00235 36186..36479 - 294 WP_004213076.1 hypothetical protein -
ENZ43_RS00240 36495..37628 - 1134 WP_004213075.1 DUF3800 domain-containing protein -
ENZ43_RS00245 38236..38466 + 231 WP_004213073.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
ENZ43_RS00250 38463..38906 + 444 WP_004213072.1 type II toxin-antitoxin system VapC family toxin Toxin
ENZ43_RS00255 38920..39336 - 417 Protein_46 restriction endonuclease -
ENZ43_RS00260 39340..39633 - 294 Protein_47 transposase -
ENZ43_RS28135 40050..40680 + 631 Protein_48 response regulator -
ENZ43_RS00275 41193..41599 - 407 Protein_49 GAF domain-containing protein -
ENZ43_RS00285 41690..42610 - 921 WP_004213934.1 DUF1471 domain-containing protein -
ENZ43_RS00290 42659..43150 - 492 WP_011251327.1 DM13 domain-containing protein -
ENZ43_RS00295 43213..43488 - 276 WP_004213932.1 carboxymuconolactone decarboxylase family protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - rmpA2 / rmpA / iutA / iucD / iucC / iucB / iucA / iroB / iroC / iroD / iroN / rmpA 1..218147 218147
- flank IS/Tn - - 34289..35257 968


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(6-129)

Antitoxin

(7-47)


Sequences


Toxin        


Download         Length: 148 a.a.        Molecular weight: 16006.74 Da        Isoelectric Point: 9.3642

>T116486 WP_004213072.1 NZ_CP034776:38463-38906 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Download         Length: 444 bp

>T116486 NZ_CP034776:38463-38906 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
TGGGCACGCTATCGCGGCCGGCGCCGTCCTAGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGAAAGCCGCTCTCTGCAGGGTAATTTCTTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8677.73 Da        Isoelectric Point: 4.4563

>AT116486 WP_004213073.1 NZ_CP034776:38236-38466 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT116486 NZ_CP034776:38236-38466 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGCTCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB Q6U619


Antitoxin

Source ID Structure
AlphaFold DB Q6U620

References