Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-DnaT
Location 48519..49041 Replicon plasmid unnamed2
Accession NZ_CP034761
Organism Klebsiella pneumoniae strain NB5306

Toxin (Protein)


Gene name relE Uniprot ID D0QMR1
Locus tag EMI85_RS26735 Protein ID WP_000220561.1
Coordinates 48760..49041 (+) Length 94 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID G3CAG1
Locus tag EMI85_RS26730 Protein ID WP_000121743.1
Coordinates 48519..48770 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EMI85_RS26705 44642..45064 - 423 WP_179292036.1 DNA distortion polypeptide 3 -
EMI85_RS26710 45132..45578 - 447 WP_001074384.1 hypothetical protein -
EMI85_RS26715 45582..46424 - 843 WP_000435064.1 replication initiation protein -
EMI85_RS26720 46445..47284 - 840 WP_024129959.1 replication initiation protein -
EMI85_RS26730 48519..48770 + 252 WP_000121743.1 plasmid stabilization protein Antitoxin
EMI85_RS26735 48760..49041 + 282 WP_000220561.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
EMI85_RS28200 49280..49372 - 93 Protein_56 IS6 family transposase -
EMI85_RS26745 49538..50737 - 1200 WP_000948429.1 IS91 family transposase -
EMI85_RS26750 50747..50935 - 189 WP_000957857.1 hypothetical protein -
EMI85_RS26755 51055..52485 + 1431 Protein_59 IS4-like element IS50R family transposase -
EMI85_RS26760 52514..53308 + 795 WP_000572405.1 aminoglycoside O-phosphotransferase APH(3')-IIa -
EMI85_RS26765 53329..53565 + 237 Protein_61 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaTEM-1B / blaCTX-M-55 / aph(3')-IIa / rmtB / floR / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / dfrA14 - 1..81092 81092


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 11023.86 Da        Isoelectric Point: 10.5938

>T116473 WP_000220561.1 NZ_CP034761:48760-49041 [Klebsiella pneumoniae]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADMR

Download         Length: 282 bp

>T116473 NZ_CP034761:48760-49041 [Klebsiella pneumoniae]
ATGACCTATGAACTGGCTTTTGACCGCAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAGCTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAAATCA
AACTTCGTGCATCTGGCTACAGACTTGTATATCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCTGTTGGAAGA
AGGGAAAGCAGCGAAGTTTATCAGATTGCAGATATGCGCTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9194.43 Da        Isoelectric Point: 4.1683

>AT116473 WP_000121743.1 NZ_CP034761:48519-48770 [Klebsiella pneumoniae]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Download         Length: 252 bp

>AT116473 NZ_CP034761:48519-48770 [Klebsiella pneumoniae]
ATGAGCTATCAAATTCTGACAACCACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3S5I302

Antitoxin

Source ID Structure
AlphaFold DB A0A3S5I301

References