Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 7394..7919 Replicon plasmid p2
Accession NZ_CP034756
Organism Enterobacter hormaechei subsp. hoffmannii strain Eh1

Toxin (Protein)


Gene name ccdB Uniprot ID W8EAA6
Locus tag EM861_RS24210 Protein ID WP_008322213.1
Coordinates 7394..7699 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A6C0NE25
Locus tag EM861_RS24215 Protein ID WP_032934863.1
Coordinates 7701..7919 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EM861_RS24180 2495..2647 + 153 Protein_2 IS5/IS1182 family transposase -
EM861_RS24185 2719..3839 + 1121 WP_089046468.1 IS3 family transposase -
EM861_RS24190 3868..4614 + 747 Protein_4 IS5 family transposase -
EM861_RS24195 4776..6098 + 1323 WP_016479957.1 GntP family transporter -
EM861_RS24200 6297..7088 - 792 WP_008322208.1 site-specific integrase -
EM861_RS24205 7078..7392 - 315 WP_008322211.1 hypothetical protein -
EM861_RS24210 7394..7699 - 306 WP_008322213.1 type II toxin-antitoxin system toxin CcdB Toxin
EM861_RS24215 7701..7919 - 219 WP_032934863.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
EM861_RS24220 8780..9477 - 698 Protein_10 IS1 family transposase -
EM861_RS24225 9519..9809 + 291 WP_045351183.1 TrbC/VirB2 family protein -
EM861_RS24230 9821..12532 + 2712 WP_015060708.1 VirB4 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaNDM-1 / sul1 / rmtC htpB 1..108486 108486
- inside IScluster/Tn - - 2531..9283 6752


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11450.23 Da        Isoelectric Point: 5.8211

>T116452 WP_008322213.1 NZ_CP034756:c7699-7394 [Enterobacter hormaechei subsp. hoffmannii]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLVSARLLSDKVSRELYPVVSVGGDPYRLMTTDMASVPATVIGEEV
ADLSHLENDIQNAINLMFRGI

Download         Length: 306 bp

>T116452 NZ_CP034756:c7699-7394 [Enterobacter hormaechei subsp. hoffmannii]
ATGCAGTTTAAGGTTTACGCCTATAAAAGAGAAAGCCGCTACCGTCTGTTCGTCGACGTGCAGAGTGACATTATTGACAC
CCCGGGACGCCGGATGGTGATCCCGCTGGTCAGCGCCCGCCTGCTGTCCGACAAAGTCTCGCGTGAGCTTTATCCGGTGG
TGTCAGTCGGAGGTGACCCTTATCGTCTGATGACCACGGATATGGCCAGTGTGCCGGCCACTGTCATCGGGGAAGAAGTG
GCCGATCTCAGCCACCTGGAGAATGACATCCAGAACGCCATCAACCTGATGTTCCGGGGGATCTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8297.11 Da        Isoelectric Point: 4.4013

>AT116452 WP_032934863.1 NZ_CP034756:c7919-7701 [Enterobacter hormaechei subsp. hoffmannii]
VKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWQEENREGMAEVASFIEANGSFADENRDW

Download         Length: 219 bp

>AT116452 NZ_CP034756:c7919-7701 [Enterobacter hormaechei subsp. hoffmannii]
GTGAAGCAGCGTATTACAGTGACAGTGGACAGCGACAGCTATCAGTTGCTCAAGGCATACGACGTAAATATCTCCGGTCT
GGTCAGTACGACCATGCAGAACGAAGCCCGCCGTCTGCGCGCCGAACGCTGGCAGGAAGAAAACCGGGAAGGTATGGCTG
AGGTGGCCAGCTTTATCGAGGCTAACGGATCGTTTGCTGACGAAAACAGGGATTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6C0NE27

Antitoxin

Source ID Structure
AlphaFold DB A0A6C0NE25

References