Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4419534..4420152 Replicon chromosome
Accession NZ_CP034738
Organism Escherichia coli strain L65

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag EM854_RS21470 Protein ID WP_001291435.1
Coordinates 4419534..4419752 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag EM854_RS21475 Protein ID WP_000344800.1
Coordinates 4419778..4420152 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EM854_RS21435 4414823..4415395 + 573 WP_000779831.1 YbaY family lipoprotein -
EM854_RS21440 4415426..4415737 - 312 WP_000409911.1 MGMT family protein -
EM854_RS21450 4416116..4416469 + 354 WP_000878140.1 DUF1428 domain-containing protein -
EM854_RS21455 4416511..4418061 - 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
EM854_RS21460 4418225..4418695 - 471 WP_000136192.1 YlaC family protein -
EM854_RS21465 4418811..4419362 - 552 WP_000102568.1 maltose O-acetyltransferase -
EM854_RS21470 4419534..4419752 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
EM854_RS21475 4419778..4420152 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
EM854_RS21480 4420698..4423847 - 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
EM854_RS21485 4423870..4425063 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T116429 WP_001291435.1 NZ_CP034738:c4419752-4419534 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T116429 NZ_CP034738:c4419752-4419534 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT116429 WP_000344800.1 NZ_CP034738:c4420152-4419778 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT116429 NZ_CP034738:c4420152-4419778 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References