Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2267604..2268258 Replicon chromosome
Accession NZ_CP034738
Organism Escherichia coli strain L65

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag EM854_RS11145 Protein ID WP_000244781.1
Coordinates 2267604..2268011 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag EM854_RS11150 Protein ID WP_000354046.1
Coordinates 2267992..2268258 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EM854_RS11125 2263561..2265294 - 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -
EM854_RS11130 2265300..2266010 - 711 WP_000715208.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
EM854_RS11135 2266035..2266931 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
EM854_RS11140 2267043..2267564 + 522 WP_001055874.1 flavodoxin FldB -
EM854_RS11145 2267604..2268011 - 408 WP_000244781.1 protein YgfX Toxin
EM854_RS11150 2267992..2268258 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
EM854_RS11155 2268501..2269481 + 981 WP_000886095.1 tRNA-modifying protein YgfZ -
EM854_RS11160 2269677..2270336 - 660 WP_000250274.1 hemolysin III family protein -
EM854_RS11165 2270500..2270811 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
EM854_RS11170 2270856..2272289 + 1434 WP_001344773.1 6-phospho-beta-glucosidase BglA -
EM854_RS11175 2272346..2273089 - 744 WP_000951961.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T116422 WP_000244781.1 NZ_CP034738:c2268011-2267604 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T116422 NZ_CP034738:c2268011-2267604 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGCTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT116422 WP_000354046.1 NZ_CP034738:c2268258-2267992 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT116422 NZ_CP034738:c2268258-2267992 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References