Detailed information of TA system TA116400

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HTH_37(antitoxin)
• Location: 2565177..2565860
• Replicon: chromosome
• Accession: NZ_CP034689
• Organism: Achromobacter spanius strain UQ283

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: ELS24_RS11930
• Protein ID: WP_127184236.1
• Coordinates: 2565177..2565362 (+)
• Length: 62 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: ELS24_RS11935
• Protein ID: WP_127184237.1
• Coordinates: 2565441..2565860 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ELS24_RS11920	2561168..2562583	+	1416	WP_127184234.1	dihydrolipoyl dehydrogenase	-
ELS24_RS11925	2562639..2564690	-	2052	WP_127184235.1	pyruvate, phosphate dikinase	-
ELS24_RS11930	2565177..2565362	+	186	WP_127184236.1	type II toxin-antitoxin system HicA family toxin	Toxin
ELS24_RS11935	2565441..2565860	+	420	WP_127184237.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
ELS24_RS11940	2565978..2567399	-	1422	WP_127184238.1	argininosuccinate lyase	-
ELS24_RS11945	2567560..2568927	+	1368	WP_050449736.1	mechanosensitive ion channel family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	acrB / ddhA / cheW / cheB / cheY	2544697..2720669	175972

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-60)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (4-62)

Sequences

Toxin

Protein

• Length: 62 a.a.
• Molecular weight: 7112.21 Da
• Isoelectric Point: 10.8600

>T116400 WP_127184236.1 NZ_CP034689:2565177-2565362 [Achromobacter spanius]
MTYSEFKRWLERQGAVFVPHRSGSSHYRVTLNGVTTIFPFHGSKEMGKHLEHQLKKQLGLK

Nucleotide

• Length: 186 bp

>T116400 NZ_CP034689:2565177-2565362 [Achromobacter spanius]
ATGACCTACAGCGAATTCAAAAGATGGCTGGAACGGCAAGGCGCGGTATTCGTACCGCATCGTTCAGGGAGTAGCCACTA
TCGTGTGACGTTAAACGGAGTGACTACGATTTTTCCATTTCACGGTTCAAAGGAAATGGGAAAACACCTGGAGCATCAGC
TCAAAAAGCAGTTGGGTTTGAAGTGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15557.79 Da
• Isoelectric Point: 4.6474

>AT116400 WP_127184237.1 NZ_CP034689:2565441-2565860 [Achromobacter spanius]
VLTYPYTLTPDTNDTLLVLFPDFPEATSVGDDEQDAAVNALEALEAALEIYFDERRPIPLPSLDRPTPYSVTLPAVVTSK
VFLSNEMIRQGVRKAEMARRLGVHMPQVDRLLNLRHSSRIEQVEAALEKLGRRLDVSIA

Nucleotide

• Length: 420 bp

>AT116400 NZ_CP034689:2565441-2565860 [Achromobacter spanius]
GTGCTGACCTATCCCTATACCCTCACTCCCGACACCAACGATACGTTGTTGGTGTTGTTTCCGGACTTCCCGGAGGCGAC
AAGTGTTGGGGATGACGAGCAAGACGCTGCCGTGAACGCCTTGGAGGCGCTTGAGGCCGCGCTGGAGATTTACTTCGACG
AGCGCCGACCCATCCCGCTGCCCAGTCTTGACCGGCCAACACCGTACTCCGTGACGTTGCCGGCCGTCGTGACGAGCAAA
GTGTTCCTGTCCAACGAGATGATCCGGCAAGGTGTGCGTAAAGCAGAGATGGCCCGCCGCTTGGGTGTTCATATGCCGCA
GGTCGATCGTCTGTTGAACCTGCGTCATTCGTCACGGATCGAGCAGGTGGAGGCGGCACTGGAGAAGCTTGGCCGTCGCC
TGGACGTATCGATCGCCTAG